Anti-Phospho-PRKAA1-T183/PRKAA2-T172 pAb Antibody (CABP0883)
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- SKU:
- CABP0883
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
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Description
| 抗体名: | Anti-Phospho-PRKAA1-T183/PRKAA2-T172 Antibody |
| 抗体コード: | CABP0883 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding T183 of human PRKAA1 |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | 293T, C6 |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding T183 of human PRKAA1 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 5562/5563 |
| Uniprot: | Q13131/P54646 |
| セルラーロケーション: | |
| 計算された分子量: | 64kDa/65kDa/62kDa |
| 観察された分子量: | 62kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (CABP0883) at 1:1000 dilution.293T and C6 cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
| 同義語: | AMPKa1/AMPKa2 |
| バックグラウンド: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |