Anti-Phospho-PRKAA1-T183/PRKAA2-T172 Antibody (CABP0116)
(No reviews yet)
Write a Review
- SKU:
- CABP0116
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-PRKAA1-T183/PRKAA2-T172 Antibody |
| 抗体コード: | CABP0116 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding T172 of human AMPKAlpha |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | C2C12 |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding T172 of human AMPKAlpha |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 5562/5563 |
| Uniprot: | Q13131/P54646 |
| セルラーロケーション: | |
| 計算された分子量: | 64kDa/65kDa |
| 観察された分子量: | 64KDa |
 | Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (CABP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37'C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (CABP0116) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (CABP0116) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (CABP0116) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKa1-T183/AMPKa2-T172 pAb (CABP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (CABP0116) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37'C for 30 minutes. |
| 同義語: | AMPKa1/AMPKa2 |
| バックグラウンド: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |