Anti-Phospho-PKCdelta/theta-S643/676 Antibody (CABP0567)
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- SKU:
- CABP0567
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
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Description
| 抗体名: | Anti-Phospho-PKCdelta/theta-S643/676 Antibody |
| 抗体コード: | CABP0567 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S643/676 of human PKCDelta/? |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | RAW264.7 |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S643/676 of human PKCDelta/? |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 5580/5588 |
| Uniprot: | Q05655/Q04759 |
| セルラーロケーション: | |
| 計算された分子量: | 77kDa/80kDa/67kDa/74kDa/81kDa |
| 観察された分子量: | 78kDa |
 | Western blot analysis of extracts of RAW264.7 cells, using Phospho-PKCdelta/theta-S643/676 antibody (CABP0567) at 1:1000 dilution. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
| 同義語: | PRKCD/PRKCQ |
| バックグラウンド: | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |