Anti-Phospho-PKC (pan) (beta-II Ser660) Antibody (CABP0495)
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- SKU:
- CABP0495
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-PKC (pan) (beta-II Ser660) Antibody |
| 抗体コード: | CABP0495 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) BetaII |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, 293T+PMA |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) BetaII |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 5578/5579/5580/5581/5583/5588 |
| Uniprot: | P17252/P05771/Q05655/Q02156/P24723/Q04759 |
| セルラーロケーション: | |
| 計算された分子量: | 59kDa/67kDa/74- 83kDa |
| 観察された分子量: | 95kDa |
 | Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (beta II Ser660) pAb (CABP0495) at 1:1000 dilution or PRKCQ antibody (CAB5834).293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
| 同義語: | PRKCA/PRKCB/PRKCD/PRKCE/PRKCH/PRKCQ |
| バックグラウンド: | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008] |