Anti-Phospho-NFKBIA (S32) Antibody (RACO0072)
- SKU:
- RACO0072
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | Phospho-NFKBIA (S32) Antibody |
| Product SKU: | RACO0072 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | WB:1:500-1:5000, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-NFKBIA (S32) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| シノニム: | NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI |
 | Immunofluorescence staining of A549 cells with RACO0072 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in A549 whole cell lysate treated with Calyculin A or not). All lanes Phospho-NFKBIA antibody at 1.07µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 39 KDa. Observed band size: 39 KDa. |
| UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P25963 |
| NCBI GenInfo Identifier: | 126682 |
| NCBI Gene ID: | 4792 |
| NCBI Accession: | P25963.1 |
| UniProt Secondary Accession: | P25963,B2R8L6, |
| UniProt Related Accession: | P25963 |
| Molecular Weight: | 35,609 Da |
| NCBI Full Name: | NF-kappa-B inhibitor alpha |
| NCBI Synonym Full Names: | NFKB inhibitor alpha |
| NCBI Official Symbol: | NFKBIA |
| NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBI |
| NCBI Protein Information: | NF-kappa-B inhibitor alpha |
| UniProt Protein Name: | NF-kappa-B inhibitor alpha |
| UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIA |
| UniProt Entry Name: | IKBA_HUMAN |