Anti-Phospho-MTOR (S2448) Antibody (RACO0130)
- SKU:
- RACO0130
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | Phospho-MTOR (S2448) Antibody |
| Product SKU: | RACO0130 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-MTOR (S2448) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity). |
| シノニム: | Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1 |
 | IHC image of RACO0130 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with RACO0130 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate A549 whole cell lysate All lanes Phospho-MTOR antibody at 0.825µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 289 KDa. Observed band size: 289 KDa. |
| UniProt Protein Function: | mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779). |
| UniProt Protein Details: | Protein type:Protein kinase, atypical; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Autophagy; EC 2.7.11.1; Kinase, protein; ATYPICAL group; PIKK family; FRAP subfamily Chromosomal Location of Human Ortholog: 1p36.2 Cellular Component: cell soma; cytoplasm; cytosol; dendrite; endomembrane system; endoplasmic reticulum membrane; Golgi membrane; lysosomal membrane; lysosome; membrane; mitochondrial outer membrane; nucleoplasm; phosphoinositide 3-kinase complex; PML body; TORC2 complex Molecular Function:ATP binding; drug binding; kinase activity; phosphoprotein binding; protein binding; protein domain specific binding; protein kinase binding; protein serine/threonine kinase activity; ribosome binding Biological Process: 'de novo' pyrimidine base biosynthetic process; brain development; cardiac muscle cell development; cardiac muscle contraction; cell aging; cell cycle arrest; cell growth; cellular response to nutrient levels; DNA repair; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; gene expression; germ cell development; growth; heart morphogenesis; innate immune response; insulin receptor signaling pathway; long-term memory; macroautophagy; maternal process involved in pregnancy; mRNA stabilization; multicellular organism growth; negative regulation of autophagy; negative regulation of cell size; negative regulation of muscle atrophy; negative regulation of NFAT protein import into nucleus; negative regulation of protein amino acid phosphorylation; negative regulation of protein ubiquitination; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; phosphorylation; positive regulation of actin filament polymerization; positive regulation of endothelial cell proliferation; positive regulation of lipid biosynthetic process; positive regulation of neuron maturation; positive regulation of nitric oxide biosynthetic process; positive regulation of oligodendrocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; post-embryonic development; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein catabolic process; regulation of actin cytoskeleton organization and biogenesis; regulation of carbohydrate utilization; regulation of fatty acid beta-oxidation; regulation of glycogen biosynthetic process; regulation of GTPase activity; regulation of myelination; regulation of osteoclast differentiation; regulation of protein kinase activity; regulation of response to food; response to amino acid stimulus; response to cocaine; response to morphine; response to nutrient; response to stress; ruffle organization and biogenesis; signal transduction; social behavior; spinal cord development; T cell costimulation; TOR signaling pathway; transcription initiation from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; visual learning; voluntary musculoskeletal movement; wound healing |
| NCBI Summary: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P42345 |
| NCBI GenInfo Identifier: | 1169735 |
| NCBI Gene ID: | 2475 |
| NCBI Accession: | P42345.1 |
| UniProt Secondary Accession: | P42345,Q4LE76, Q5TER1, Q6LE87, Q96QG3, Q9Y4I3, |
| UniProt Related Accession: | P42345 |
| Molecular Weight: | 288,892 Da |
| NCBI Full Name: | Serine/threonine-protein kinase mTOR |
| NCBI Synonym Full Names: | mechanistic target of rapamycin |
| NCBI Official Symbol: | MTOR |
| NCBI Official Synonym Symbols: | SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1 |
| NCBI Protein Information: | serine/threonine-protein kinase mTOR |
| UniProt Protein Name: | Serine/threonine-protein kinase mTOR |
| UniProt Synonym Protein Names: | FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | MTOR |
| UniProt Entry Name: | MTOR_HUMAN |