Anti-Phospho-LAT-Y171 pAb Antibody (CABP0809)
- SKU:
- CABP0809
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-LAT-Y171 Antibody |
| 抗体コード: | CABP0809 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | Jurkat |
| 免疫原: | A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DDYV N |
| 遺伝子ID: | 27040 |
| Uniprot: | O43561 |
| セルラーロケーション: | Cell membrane, Single-pass type III membrane protein |
| 計算された分子量: | 24kDa/27kDa/28kDa |
| 観察された分子量: | 38kDa |
 | Western blot analysis of extracts of Jurkat cells, using Phospho-LAT-Y171 antibody (CABP0809) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 ug/mL) for 2 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
| 同義語: | LAT, LAT1, pp36, IMD52 |
| バックグラウンド: | The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. |
| NCBI Summary: | The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43561 |
| NCBI GenInfo Identifier: | 14194891 |
| NCBI Gene ID: | 27040 |
| NCBI Accession: | O43561.1 |
| UniProt Secondary Accession: | O43561,O43919, B7WPI0, C7C5T6, G5E9K3, |
| UniProt Related Accession: | O43561 |
| Molecular Weight: | 27,774 Da |
| NCBI Full Name: | Linker for activation of T-cells family member 1 |
| NCBI Synonym Full Names: | linker for activation of T-cells |
| NCBI Official Symbol: | LAT |
| NCBI Official Synonym Symbols: | LAT1; pp36 |
| NCBI Protein Information: | linker for activation of T-cells family member 1 |
| UniProt Protein Name: | Linker for activation of T-cells family member 1 |
| UniProt Synonym Protein Names: | 36 kDa phospho-tyrosine adapter protein; pp36; p36-38 |
| Protein Family: | Latexin |
| UniProt Gene Name: | LAT |
| UniProt Entry Name: | LAT_HUMAN |