Anti-Phospho-IKK alpha/beta-S176/180 pAb Antibody (CABP0891)
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- SKU:
- CABP0891
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-IKK alpha/beta-S176/180 Antibody |
| 抗体コード: | CABP0891 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S176/180 of human IKKAlpha/Beta. |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S176/180 of human IKKAlpha/Beta. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 1147/3551 |
| Uniprot: | |
| セルラーロケーション: | |
| 計算された分子量: | |
| 観察された分子量: | 85kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-IKKa/b-S176/180 antibody (CABP0891) at 1:1000 dilution.HeLa cells were treated by TNF-a (20 ng/mL) and Calyculin A (100 nM) at 37'C for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| 同義語: | |
| バックグラウンド: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008] |