Anti-Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 pAb Antibody (CABP0902)
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- SKU:
- CABP0902
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
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Description
| 抗体名: | Anti-Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 Antibody |
| 抗体コード: | CABP0902 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding Y1165/1166 of human INSR and Y1131 of human IGF1R |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding Y1165/1166 of human INSR and Y1131 of human IGF1R |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 3480/3643 |
| Uniprot: | P08069/P06213 |
| セルラーロケーション: | |
| 計算された分子量: | 154kDa/155kDa/156kDa |
| 観察された分子量: | 125kDa |
 | Western blot analysis of extracts of HeLa cells, using Anti-Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 pAb (CABP0902) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| 同義語: | IGF1R/INSR |
| バックグラウンド: | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |