Anti-Phospho-GSK3A-S21 Antibody (CABP0582)
- SKU:
- CABP0582
- Product type:
- Antibody
- Application:
- WB
- Application:
- IP
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-GSK3A-S21 Antibody |
| 抗体コード: | CABP0582 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IP |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around S21 of human GSK3Alpha (NP_063937.2). |
| 申し込み: | WB IP |
| 推奨希釈: | WB 1:500 - 1:2000 IP 1:50 - 1:100 |
| 反応性: | Human |
| ポジティブサンプル: | 293, HeLa |
| 免疫原: | A synthetic phosphorylated peptide around S21 of human GSK3Alpha (NP_063937.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TSSF A |
| 遺伝子ID: | 2931 |
| Uniprot: | P49840 |
| セルラーロケーション: | |
| 計算された分子量: | 50kDa |
| 観察された分子量: | 51kDa |
 | Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3a-S21 antibody (CABP0582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Western blot analysis of extracts of 293 cells, using Phospho-GSK3a-S21 antibody (CABP0582) at 1:1000 dilution or GSK3a antibody (CAB0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3a-S21 pAb (CABP0582). Western blot was performed from the immunoprecipitate using Phospho-GSK3a-S21 pAb (CABP0582) at a dilition of 1:1000. 293T cells were treated by Insulin (100 nM) at 37'C for 10 minutes after serum-starvation overnight. |
| 同義語: | GSK3 alpha, GSK3A |
| バックグラウンド: | This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. |
| UniProt Protein Function: | GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418 |
| UniProt Protein Details: | Protein type:EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; GSK family; GSK subfamily Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: beta-catenin destruction complex; cytosol Molecular Function:protein binding; protein serine/threonine kinase activity; tau-protein kinase activity Biological Process: cellular response to insulin stimulus; insulin receptor signaling pathway; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling pathway; positive regulation of cAMP biosynthetic process; positive regulation of heart contraction; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure |
| NCBI Summary: | This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P49840 |
| NCBI GenInfo Identifier: | 12644292 |
| NCBI Gene ID: | 2931 |
| NCBI Accession: | P49840.2 |
| UniProt Secondary Accession: | P49840,O14959, |
| UniProt Related Accession: | P49840 |
| Molecular Weight: | 50,981 Da |
| NCBI Full Name: | Glycogen synthase kinase-3 alpha |
| NCBI Synonym Full Names: | glycogen synthase kinase 3 alpha |
| NCBI Official Symbol: | GSK3A |
| NCBI Protein Information: | glycogen synthase kinase-3 alpha |
| UniProt Protein Name: | Glycogen synthase kinase-3 alpha |
| UniProt Synonym Protein Names: | Serine/threonine-protein kinase GSK3A (EC:2.7.11.1) |
| Protein Family: | Glycogen synthase kinase |
| UniProt Gene Name: | GSK3A |
| UniProt Entry Name: | GSK3A_HUMAN |
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