Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody (CABP0472)
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- SKU:
- CABP0472
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody |
| 抗体コード: | CABP0472 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3). |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, NIH/3T3, C6 |
| 免疫原: | A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FLTE YVA |
| 遺伝子ID: | 5594/5595 |
| Uniprot: | P28482/P27361 |
| セルラーロケーション: | |
| 計算された分子量: | 36kDa/41kDa/38kDa/40kDa/43kDa |
| 観察された分子量: | 42KDa/44KDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (CABP0472) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human colon using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody (CABP0472). Western blot was performed from the immunoprecipitate using Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody (CABP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37'C for 10 minutes. |
| 同義語: | MAPK1/MAPK3 |
| バックグラウンド: | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] |