Anti-Phospho-eIF2a-S49 Antibody (CABP0341)
- SKU:
- CABP0341
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
抗体名: | Anti-Phospho-eIF2a-S49 Antibody |
抗体コード: | CABP0341 |
抗体サイズ: | 50uL, 100uL |
申し込み: | WB IHC IF |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | A phospho specific peptide corresponding to residues surrounding S49 of human EIF2S1 |
申し込み: | WB IHC IF |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | HeLa |
免疫原: | A phospho specific peptide corresponding to residues surrounding S49 of human EIF2S1 |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | Email for sequence |
遺伝子ID: | 1965 |
Uniprot: | P05198 |
セルラーロケーション: | Cytoplasmic granule |
計算された分子量: | 36kDa |
観察された分子量: | 40kDa |
同義語: | EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1 |
バックグラウンド: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
UniProt Protein Function: | eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections. |
UniProt Protein Details: | Protein type:RNA-binding; Translation initiation; Translation Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex Molecular Function:protein binding; translation initiation factor activity; ribosome binding Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression |
NCBI Summary: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
UniProt Code: | P05198 |
NCBI GenInfo Identifier: | 124200 |
NCBI Gene ID: | 1965 |
NCBI Accession: | P05198.3 |
UniProt Related Accession: | P05198 |
Molecular Weight: | 315 |
NCBI Full Name: | Eukaryotic translation initiation factor 2 subunit 1 |
NCBI Synonym Full Names: | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
NCBI Official Symbol: | EIF2S1 |
NCBI Official Synonym Symbols: | EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha |
NCBI Protein Information: | eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha |
UniProt Protein Name: | Eukaryotic translation initiation factor 2 subunit 1 |
UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha |
Protein Family: | Eukaryotic translation initiation factor |
UniProt Gene Name: | EIF2S1 |
UniProt Entry Name: | IF2A_HUMAN |