Anti-Phospho-CREB1-S133 Antibody (CABP0019)
- SKU:
- CABP0019
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Application:
- IP
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-Phospho-CREB1-S133 Antibody |
| 抗体コード: | CABP0019 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1). |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | HeLa |
| 免疫原: | A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RPSY R |
| 遺伝子ID: | 1385 |
| Uniprot: | P16220 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 25kDa/35kDa/36kDa |
| 観察された分子量: | 36kDa |
 | Western blot analysis of extracts of HeLa cells, using Anti-Phospho-CREB1-S133 Antibody (CABP0019) at 1:2000 dilution or CREB1 antibody (CAB11064).HeLa cells were treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human appendix using Phospho-CREB1-S133 antibody (CABP0019) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal using Phospho-CREB1-S133 antibody (CABP0019) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 antibody (CABP0019). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Anti-Phospho-CREB1-S133 Antibody (CABP0019). Western blot was performed from the immunoprecipitate using Anti-Phospho-CREB1-S133 Antibody (CABP0019) at a dilition of 1:1000. 293T cells were treated by 10% FBS at 37'C for 30 minutes after serum-starvation overnight. |
| 同義語: | CREB1, CREB, CREB-1 |
| バックグラウンド: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. |
| UniProt Protein Function: | Function: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. |
| UniProt Protein Details: | Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity. Interacts (phosporylated form) with TOX3. Interacts with ARRB1. Ref.8 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.20 Subcellular location: Nucleus Ref.12. Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.17 Ref.19Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Ref.12 Involvement in Disease: Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [ MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP domain.Contains 1 KID (kinase-inducible) domain. |
| NCBI Summary: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| UniProt Code: | P16220 |
| NCBI GenInfo Identifier: | 117434 |
| NCBI Gene ID: | 1385 |
| NCBI Accession: | P16220.2 |
| UniProt Secondary Accession: | P16220,P21934, Q9UMA7, |
| UniProt Related Accession: | P16220,Q16366,Q16367,Q4ZG78,Q53RU9,Q53X93,Q5U0J5,Q6V963 |
| Molecular Weight: | 40 kDa |
| NCBI Full Name: | Cyclic AMP-responsive element-binding protein 1 |
| NCBI Synonym Full Names: | cAMP responsive element binding protein 1 |
| NCBI Official Symbol: | CREB1 |
| NCBI Official Synonym Symbols: | CREB; MGC9284 |
| NCBI Protein Information: | cyclic AMP-responsive element-binding protein 1; CREB-1; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1 |
| UniProt Protein Name: | Cyclic AMP-responsive element-binding protein 1 |
| Protein Family: | CREB-binding protein |
| UniProt Gene Name: | CREB1 |
| UniProt Entry Name: | CREB1_HUMAN |