Anti-Phospho-CDK4-T172 Antibody (CABP0593)
- SKU:
- CABP0593
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| 抗体名: | Anti-Phospho-CDK4-T172 Antibody |
| 抗体コード: | CABP0593 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | NIH/3T3 |
| 免疫原: | A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ALTP V |
| 遺伝子ID: | 1019 |
| Uniprot: | P11802 |
| セルラーロケーション: | Cytoplasm, Membrane, Nucleus |
| 計算された分子量: | 20kDa/33kDa |
| 観察された分子量: | 33KDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Anti-Phospho-CDK4-T172 Antibody (CABP0593) at 1:1000 dilution or CDK4 antibody (CAB0366).NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| 同義語: | CDK4, CMM3, PSK-J3 |
| バックグラウンド: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. |
| UniProt Protein Function: | CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991. |
| UniProt Protein Details: | Protein type:EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily Chromosomal Location of Human Ortholog: 12q14 Cellular Component: nucleoplasm; transcription factor complex; nuclear membrane; tight junction; perinuclear region of cytoplasm; nucleolus; cyclin-dependent protein kinase holoenzyme complex; nucleus; chromatin; cytosol Molecular Function:protein binding; cyclin binding; cyclin-dependent protein kinase activity; protein complex binding; cyclin-dependent protein kinase regulator activity; ATP binding Biological Process: circadian rhythm; lens development in camera-type eye; response to drug; establishment and/or maintenance of chromatin architecture; organ regeneration; positive regulation of cell size; positive regulation of translation; positive regulation of apoptosis; response to toxin; response to testosterone stimulus; signal transduction; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; response to hyperoxia; regulation of gene expression; cell division; response to lead ion; positive regulation of cell proliferation; mitotic cell cycle; regulation of protein kinase activity; G1/S transition of mitotic cell cycle Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3 |
| NCBI Summary: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11802 |
| NCBI GenInfo Identifier: | 1168867 |
| NCBI Gene ID: | 1019 |
| NCBI Accession: | P11802.2 |
| UniProt Secondary Accession: | P11802,O00576, Q6FG61, B2R9A0, B4DNF9, |
| UniProt Related Accession: | P11802 |
| Molecular Weight: | Calculated MW: 20kDa/33kDaObserved MW: 34kDa |
| NCBI Full Name: | Cyclin-dependent kinase 4 |
| NCBI Synonym Full Names: | cyclin-dependent kinase 4 |
| NCBI Official Symbol: | CDK4 |
| NCBI Official Synonym Symbols: | CMM3; PSK-J3 |
| NCBI Protein Information: | cyclin-dependent kinase 4; cell division protein kinase 4 |
| UniProt Protein Name: | Cyclin-dependent kinase 4 |
| UniProt Synonym Protein Names: | Cell division protein kinase 4; PSK-J3 |
| Protein Family: | Cyclin-dependent kinase |
| UniProt Gene Name: | CDK4 |
| UniProt Entry Name: | CDK4_HUMAN |