Anti-Phospho-CAD-S1859 pAb Antibody (CABP0884)
- SKU:
- CABP0884
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
抗体名: | Anti-Phospho-CAD-S1859 Antibody |
抗体コード: | CABP0884 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB |
反応性: | Human |
宿主種: | Rabbit |
免疫原: | A synthetic phosphorylated peptide around S1859 of human CAD (NP_004332.2). |
申し込み: | WB |
推奨希釈: | WB 1:500 - 1:2000 |
反応性: | Human |
ポジティブサンプル: |
免疫原: | A synthetic phosphorylated peptide around S1859 of human CAD (NP_004332.2). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | RASD P |
遺伝子ID: | 790 |
Uniprot: | P27708 |
セルラーロケーション: | Cytoplasm, Nucleus |
計算された分子量: | 242kDa |
観察された分子量: | Refer to figures |
同義語: | CAD, CDG1Z, EIEE50, GATD4 |
バックグラウンド: | The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. |
UniProt Protein Function: | CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine. |
UniProt Protein Details: | Protein type:EC 3.5.2.3; EC 6.3.5.5; Nucleotide Metabolism - pyrimidine; Amino Acid Metabolism - alanine, aspartate and glutamate; Ligase; Hydrolase; Transferase; EC 2.1.3.2 Chromosomal Location of Human Ortholog: 2p22-p21 Cellular Component: cell projection; cell soma; cytosol; membrane; nuclear matrix; nucleoplasm; nucleus; terminal button Molecular Function:aspartate carbamoyltransferase activity; ATP binding; carbamoyl-phosphate synthase (ammonia) activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; dihydroorotase activity; enzyme binding; identical protein binding; protein kinase activity; zinc ion binding Biological Process: 'de novo' pyrimidine base biosynthetic process; arginine biosynthetic process; drug metabolic process; glutamine metabolic process; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; pyrimidine nucleoside biosynthetic process; urea cycle Disease: Congenital Disorder Of Glycosylation, Type Iz |
NCBI Summary: | The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
UniProt Code: | P27708 |
NCBI GenInfo Identifier: | 50403731 |
NCBI Gene ID: | 790 |
NCBI Accession: | P27708.3 |
UniProt Secondary Accession: | P27708,Q6P0Q0, Q96CK3, D6W552, |
UniProt Related Accession: | P27708 |
Molecular Weight: | 242,984 Da |
NCBI Full Name: | CAD protein |
NCBI Synonym Full Names: | carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
NCBI Official Symbol: | CAD |
NCBI Official Synonym Symbols: | CDG1Z |
NCBI Protein Information: | CAD protein |
UniProt Protein Name: | CAD protein |
Protein Family: | Cadherin |
UniProt Gene Name: | CAD |
UniProt Entry Name: | PYR1_HUMAN |