Anti-PFKFB3 Antibody (CAB6945)
- SKU:
- CAB6945
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PFKFB3 Antibody |
| 抗体コード: | CAB6945 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, SKOV3 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LDKS AEEM PYLK CPLH TVLK LTPV AYGC RVES IYLN VESV CTHR ERSE DAKK GPNP LMRR NSVT PLAS PEPT KKPR INSF EEHV ASTS AALP SCLP PEVP T |
| 遺伝子ID: | 5209 |
| Uniprot: | Q16875 |
| セルラーロケーション: | |
| 計算された分子量: | 57kDa/58kDa/59kDa/60kDa |
| 観察された分子量: | 57kDa |
| 同義語: | PFKFB3, IPFK2, PFK2, iPFK-2 |
| バックグラウンド: | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2, 6-bisphosphate (F2, 6BP), and a fructose-2, 6-biphosphatase activity that catalyzes the degradation of F2, 6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PFKFB3: Synthesis and degradation of fructose 2,6-bisphosphate. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, other; Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.46; Phosphatase (non-protein); EC 2.7.1.105 Chromosomal Location of Human Ortholog: 10p15.1 Cellular Component: cytosol; nucleoplasm Molecular Function:6-phosphofructo-2-kinase activity |
| NCBI Summary: | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] |
| UniProt Code: | Q16875 |
| NCBI GenInfo Identifier: | 3023733 |
| NCBI Gene ID: | 5209 |
| NCBI Accession: | Q16875.1 |
| UniProt Secondary Accession: | Q16875,O43622, O75902, Q5VX15, Q5VX18, Q5VX19, B7Z955 |
| UniProt Related Accession: | Q16875 |
| Molecular Weight: | 60,602 Da |
| NCBI Full Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 |
| NCBI Synonym Full Names: | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 |
| NCBI Official Symbol: | PFKFB3 |
| NCBI Official Synonym Symbols: | PFK2; IPFK2; iPFK-2 |
| NCBI Protein Information: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 |
| UniProt Protein Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 |
| UniProt Synonym Protein Names: | 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2Including the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105)Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
| Protein Family: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase |
| UniProt Gene Name: | PFKFB3 |
| UniProt Entry Name: | F263_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFKFB3 antibody (CAB6945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using PFKFB3 Rabbit pAb (CAB6945) at dilution of 1:25 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using PFKFB3 Rabbit pAb (CAB6945) at dilution of 1:25 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using PFKFB3 Rabbit pAb (CAB6945) at dilution of 1:25 (40x lens). |
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