Anti-PEX14 抗体 (CAB7336)

抗体名:	Anti-PEX14 Antibody
抗体コード:	CAB7336
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse
ポジティブサンプル:	Mouse liver

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	GIAF GFHQ LYKK YLLP LILG GRED RKQL ERME AGLS ELSG SVAQ TVTQ LQTT LASV QELL IQQQ QKIQ ELAH ELAA AKAT TSTN WILE SQNI NELK SEIN SLKG LLLN RRQF PPSP SAPK IPSW QIPV KSPS PSSP AAVN HHSS SDIS PVSN ESTS SSPG KEGH SPEG STVT YHLL GPQE EGEG VVDV KGQV RMEV QGEE EKRE DKED EEDE EDDD VSHV DEED CLGV QRED RRGG DGQI NEQV EKLR RPEG ASNE SERD
遺伝子ID:	5195
Uniprot:	O75381
セルラーロケーション:	Cytoplasmic side, Peripheral membrane protein, Peroxisome membrane
計算された分子量:	36kDa/41kDa
観察された分子量:	41kDa

同義語:

PEX14, NAPP2, PBD13A, Pex14p, dJ734G22.2

バックグラウンド:

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.

UniProt Protein Function:	PEX14: Component of the peroxisomal translocation machinery with PEX13 and PEX17. Interacts with both the PTS1 and PTS2 receptors. Binds directly to PEX17. Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). PBD-CGK is a peroxisomal disorder arising from a failure of protein import into the peroxisomal membrane or matrix. The peroxisome biogenesis disorders (PBD group) are genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Include disorders are: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum (PBD- ZSS). Defects in PEX14 are a cause of Zellweger syndrome (ZWS). ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. Belongs to the peroxin-14 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: integral to membrane; intracellular; intracellular membrane-bound organelle; membrane; nucleolus; peroxisomal membrane; peroxisome; protein complex Molecular Function:beta-tubulin binding; microtubule binding; protein binding; protein N-terminus binding; receptor binding; transcription corepressor activity Biological Process: negative regulation of protein binding; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; peroxisome organization and biogenesis; protein complex assembly; protein homooligomerization; protein import into peroxisome matrix; protein import into peroxisome matrix, translocation Disease: Peroxisome Biogenesis Disorder 13a (zellweger)
NCBI Summary:	This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:	O75381
NCBI GenInfo Identifier:	6919940
NCBI Gene ID:	5195
NCBI Accession:	O75381.1
UniProt Secondary Accession:	O75381,Q8WX51, B2R7N1, B3KML6, B7Z1N2,
UniProt Related Accession:	O75381
Molecular Weight:	36,702 Da
NCBI Full Name:	Peroxisomal membrane protein PEX14
NCBI Synonym Full Names:	peroxisomal biogenesis factor 14
NCBI Official Symbol:	PEX14
NCBI Official Synonym Symbols:	NAPP2; PBD13A; Pex14p; dJ734G22.2
NCBI Protein Information:	peroxisomal membrane protein PEX14
UniProt Protein Name:	Peroxisomal membrane protein PEX14
UniProt Synonym Protein Names:	PTS1 receptor-docking protein; Peroxin-14; Peroxisomal membrane anchor protein PEX14
Protein Family:	Peroxisomal membrane protein
UniProt Gene Name:	PEX14
UniProt Entry Name:	PEX14_HUMAN

	Western blot analysis of extracts of mouse liver, using PEX14 antibody (CAB7336) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate cancer using PEX14 antibody (CAB7336) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using PEX14 antibody (CAB7336) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using PEX14 antibody (CAB7336). Blue: DAPI for nuclear staining.

Antibodies

PEX14 Antibody (PACO01319)

PEX14 Antibody (PACO11174)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)