Anti-PEDF Antibody (CAB1673)
- SKU:
- CAB1673
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PEDF Antibody |
| 抗体コード: | CAB1673 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PEDF (NP_002606.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HepG2, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PEDF (NP_002606.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MQAL VLLL CIGA LLGH SSCQ NPAS PPEE GSPD PDST GALV EEED PFFK VPVN KLAA AVSN FGYD LYRV RSST SPTT NVLL SPLS VATA LSAL SLGA EQRT ESII HRAL YYDL ISSP DIHG TYKE LLDT VTAP QKNL KSAS RIVF EKKL RIKS SFVA PLEK SYGT RPRV LTGN PRLD LQEI NNWV QAQM KGKL ARST KEIP |
| 遺伝子ID: | 5176 |
| Uniprot: | P36955 |
| セルラーロケーション: | Melanosome, Secreted |
| 計算された分子量: | 46kDa |
| 観察された分子量: | 46KDa |
| 同義語: | SERPINF1, EPC-1, OI12, OI6, PEDF, PIG35 |
| バックグラウンド: | The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. |
| UniProt Protein Function: | PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Inhibitor Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane Molecular Function:serine-type endopeptidase inhibitor activity Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; multicellular organismal development; negative regulation of inflammatory response; response to acidity; kidney development; aging Disease: Osteogenesis Imperfecta, Type Vi |
| NCBI Summary: | The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011] |
| UniProt Code: | P36955 |
| NCBI GenInfo Identifier: | 313104314 |
| NCBI Gene ID: | 5176 |
| NCBI Accession: | P36955.4 |
| UniProt Secondary Accession: | P36955,Q13236, Q2TU83, Q96CT1, Q96R01, Q9BWA4, F1T092 |
| UniProt Related Accession: | P36955 |
| Molecular Weight: | 46,312 Da |
| NCBI Full Name: | Pigment epithelium-derived factor |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 |
| NCBI Official Symbol: | SERPINF1 |
| NCBI Official Synonym Symbols: | OI6; OI12; PEDF; EPC-1; PIG35 |
| NCBI Protein Information: | pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 |
| UniProt Protein Name: | Pigment epithelium-derived factor |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1 |
| UniProt Gene Name: | SERPINF1 |
| UniProt Entry Name: | PEDF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PEDF antibody (CAB1673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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