Anti-PDZD3 Antibody (CAB12222)
- SKU:
- CAB12222
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PDZD3 Antibody |
| 抗体コード: | CAB12222 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PDZD3 (NP_079067.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, SGC-7901, HT-29, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PDZD3 (NP_079067.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEKA ADLQ DTAS LTLK FKFN PKLG IDNP VLSL AEDH DPYD PWSL ERPR FCLL SKEE GKSF GFHL QQEL GRAG HVVC RVDP GTSA QRQG LQEG DRIL AVNN DVVE HEDY AVVV RRIR ASSP RVLL TVLA RHAH DVAR AQLG EDAH LCPT LGPG VRPR LCHI VKDE GGFG FSVT HGNQ GPFW |
| 遺伝子ID: | 79849 |
| Uniprot: | Q86UT5 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Peripheral membrane protein |
| 計算された分子量: | 24kDa/41kDa/52kDa/54kDa/61kDa |
| 観察された分子量: | 56kDa |
| 同義語: | PDZD3, IKEPP, NHERF4, PDZK2 |
| バックグラウンド: |
| UniProt Protein Function: | PDZD3: Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. Stimulates SLC9A3 activity in the presence of elevated calcium ions. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: apical part of cell; brush border; cytosol; subapical complex Molecular Function:guanylate cyclase inhibitor activity; ion channel inhibitor activity; protein binding; protein C-terminus binding Biological Process: negative regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to toxin |
| NCBI Summary: | Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q86UT5 |
| NCBI GenInfo Identifier: | 308153467 |
| NCBI Gene ID: | 79849 |
| NCBI Accession: | Q86UT5.2 |
| UniProt Secondary Accession: | Q86UT5,Q8N6R4, Q8NAW7, Q8NEX7, Q9H5Z3, |
| UniProt Related Accession: | Q86UT5 |
| Molecular Weight: | 24,105 Da |
| NCBI Full Name: | Na(+)/H(+) exchange regulatory cofactor NHE-RF4 |
| NCBI Synonym Full Names: | PDZ domain containing 3 |
| NCBI Official Symbol: | PDZD3 |
| NCBI Official Synonym Symbols: | IKEPP; PDZK2; NHERF4 |
| NCBI Protein Information: | Na(+)/H(+) exchange regulatory cofactor NHE-RF4 |
| UniProt Protein Name: | Na(+)/H(+) exchange regulatory cofactor NHE-RF4 |
| UniProt Synonym Protein Names: | Intestinal and kidney-enriched PDZ protein; Natrium-phosphate cotransporter IIa C-terminal-associated protein 2; Na/Pi cotransporter C-terminal-associated protein 2; NaPi-Cap2; PDZ domain-containing protein 2; PDZ domain-containing protein 3; Sodium-hydrogen exchanger regulatory factor 4 |
| Protein Family: | Na(+)/H(+) exchange regulatory cofactor |
| UniProt Gene Name: | PDZD3 |
| UniProt Entry Name: | NHRF4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDZD3 antibody (CAB12222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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