Anti-PDP1 Antibody (CAB14545)
- SKU:
- CAB14545
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PDP1 Antibody |
| 抗体コード: | CAB14545 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 72-260 of human PDP1 (NP_060914.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, U-87MG, DU145, 5637, mouse brain, mouse skeletal muscle, mouse heart, rat skeletal muscle, rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 72-260 of human PDP1 (NP_060914.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ASTP QKFY LTPP QVNS ILKA NEYS FKVP EFDG KNVS SILG FDSN QLPA NAPI EDRR SAAT CLQT RGML LGVF DGHA GCAC SQAV SERL FYYI AVSL LPHE TLLE IENA VESG RALL PILQ WHKH PNDY FSKE ASKL YFNS LRTY WQEL IDLN TGES TDID VKEA LINA FKRL DNDI SLEA QVGD PNSF L |
| 遺伝子ID: | 54704 |
| Uniprot: | Q9P0J1 |
| セルラーロケーション: | Mitochondrion matrix |
| 計算された分子量: | 61kDa/63kDa |
| 観察された分子量: | 61kDa |
| 同義語: | PDP1, PDH, PDP, PDPC, PPM2A, PPM2C |
| バックグラウンド: | Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | PDP1: a protein phosphoserine phosphatase associated with the mitochondrial matrix that activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation. The PDPs play crucial roles in switching metabolic flux from glycolysis towards oxidative phosphorylation. Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. PDP1 is composed of a catalytic subunit and a regulatory subunit (PDPR). Heterodimer of a catalytic subunit and a FAD protein of unknown function. Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency). PDP deficiency results in lactic acidosis leading to neurological dysfunction. Belongs to the PP2C family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 3.1.3.43; Mitochondrial; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 8q22.1 Cellular Component: mitochondrial matrix Molecular Function:[pyruvate dehydrogenase (lipoamide)] phosphatase activity; protein binding; protein serine/threonine phosphatase activity Biological Process: regulation of acetyl-CoA biosynthetic process from pyruvate Disease: Pyruvate Dehydrogenase Phosphatase Deficiency |
| NCBI Summary: | Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009] |
| UniProt Code: | Q9P0J1 |
| NCBI GenInfo Identifier: | 78099789 |
| NCBI Gene ID: | 54704 |
| NCBI Accession: | Q9P0J1.3 |
| UniProt Secondary Accession: | Q9P0J1,Q5U5K1, B3KX71, J3KPU0, |
| UniProt Related Accession: | Q9P0J1 |
| Molecular Weight: | 63,695 Da |
| NCBI Full Name: | [Pyruvate dehydrogenase |
| NCBI Synonym Full Names: | pyruvate dehyrogenase phosphatase catalytic subunit 1 |
| NCBI Official Symbol: | PDP1 |
| NCBI Official Synonym Symbols: | PDH; PDP; PDPC; PPM2A; PPM2C |
| NCBI Protein Information: | pyruvate dehyrogenase phosphatase catalytic subunit 1 |
| UniProt Protein Name: | [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial |
| UniProt Synonym Protein Names: | Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1 |
| Protein Family: | PWWP domain-containing protein |
| UniProt Gene Name: | PDP1 |
| UniProt Entry Name: | PDP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDP1 antibody (CAB14545) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using PDP1 Rabbit pAb (CAB14545) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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