Anti-PDLIM5 Antibody (CAB5720)
- SKU:
- CAB5720
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PDLIM5 Antibody |
| 抗体コード: | CAB5720 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HL-60, BT-474, HepG2, MCF7, Mouse lung, Mouse heart, Mouse kidney, Mouse uterus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ASSV ASTR SMPE SLDS PTSG RPGV TSLT TAAA FKPV GSTG VIKS PSWQ RPNQ GVPS TGRI SNSA TYSG SVAP ANSA LGQT QPSD QDTL VQRA EHIP AGKR TPMC AHCN QVIR GPFL VALG KSWH PEEF NCAH CKNT MAYI GFVE EKGA LYCE LCYE KFFA PECG RCQR KILG EVIS ALKQ TWHV SCFV CVAC GKPI RNNV FHLE DGEP YCET DYYA LFGT ICHG CEFP IEAG DMFL EALG YTWH DTCF VCSV CCES LEGQ TFFS KKDK PLCK KHAH SVNF |
| 遺伝子ID: | 10611 |
| Uniprot: | Q96HC4 |
| セルラーロケーション: | Cell junction, Cytoplasm, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome |
| 計算された分子量: | 15kDa/23kDa/25kDa/52kDa/53kDa/63kDa/67kDa |
| 観察された分子量: | 68kDa |
| 同義語: | PDLIM5, ENH, ENH1, L9, LIM |
| バックグラウンド: | This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | LIM: an adaptor protein of the Enigma family. Contains three LIM domains and one PDZ domain. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. Enigma family proteins possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 4q22 Cellular Component: postsynaptic membrane; neuron projection; membrane; postsynaptic density; cell junction; Z disc; cytosol; actin cytoskeleton Molecular Function:actinin binding; protein kinase C binding; zinc ion binding; protein N-terminus binding; actin binding Biological Process: regulation of synaptogenesis |
| NCBI Summary: | This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| UniProt Code: | Q96HC4 |
| NCBI GenInfo Identifier: | 317373590 |
| NCBI Gene ID: | 10611 |
| NCBI Accession: | Q96HC4.5 |
| UniProt Secondary Accession: | Q96HC4,O60705, Q56VN4, Q5UW38, Q8WVK0, A8K6F9, D6RB78 E9PBF5, |
| UniProt Related Accession: | Q96HC4 |
| Molecular Weight: | 63,945 Da |
| NCBI Full Name: | PDZ and LIM domain protein 5 |
| NCBI Synonym Full Names: | PDZ and LIM domain 5 |
| NCBI Official Symbol: | PDLIM5 |
| NCBI Official Synonym Symbols: | L9; ENH; LIM; ENH1 |
| NCBI Protein Information: | PDZ and LIM domain protein 5; enigma homolog; enigma-like LIM domain protein; enigma-like PDZ and LIM domains protein |
| UniProt Protein Name: | PDZ and LIM domain protein 5 |
| UniProt Synonym Protein Names: | Enigma homolog; Enigma-like PDZ and LIM domains protein |
| UniProt Gene Name: | PDLIM5 |
| UniProt Entry Name: | PDLI5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDLIM5 antibody (CAB5720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using PDLIM5 antibody (CAB5720). Blue: DAPI for nuclear staining. |
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