Anti-PDIA3 Antibody (CAB1085)

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SKU:
CAB1085
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-PDIA3 Antibody
抗体コード:CAB1085
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 206-505 of human PDIA3 (NP_005304.3).
申し込み:WB IHC IF
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:Human, Mouse, Rat
ポジティブサンプル:NIH/3T3, MCF7, Jurkat, HepG2, LO2, Mouse brain, Mouse thymus, Mouse liver, Rat liver
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 206-505 of human PDIA3 (NP_005304.3).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:FRPS HLTN KFED KTVA YTEQ KMTS GKIK KFIQ ENIF GICP HMTE DNKD LIQG KDLL IAYY DVDY EKNA KGSN YWRN RVMM VAKK FLDA GHKL NFAV ASRK TFSH ELSD FGLE STAG EIPV VAIR TAKG EKFV MQEE FSRD GKAL ERFL QDYF DGNL KRYL KSEP IPES NDGP VKVV VAEN FDEI VNNE NKDV LIEF YAPW CGHC KNLE PKYK ELGE KLSK DPNI VIAK MDAT ANDV PSPY EVRG FPTI YFSP ANKK LNPK KYEG GREL SDFI SYLQ REAT NPPV IQEE KPKK KKKA QEDL
遺伝子ID:2923
Uniprot:P30101
セルラーロケーション:Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome
計算された分子量:56kDa
観察された分子量:57kDa
同義語:PDIA3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC
バックグラウンド:This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates.
UniProt Protein Function:GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.
UniProt Protein Details:

Protein type:Chaperone; Endoplasmic reticulum; Protease; Isomerase; EC 5.3.4.1; Apoptosis; Phospholipase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: smooth endoplasmic reticulum; cell surface; focal adhesion; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; nucleus

Molecular Function:disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER

NCBI Summary:This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
UniProt Code:P30101
NCBI GenInfo Identifier:2507461
NCBI Gene ID:2923
NCBI Accession:P30101.4
UniProt Secondary Accession:P30101,Q13453, Q14255, Q8IYF8, Q9UMU7,
UniProt Related Accession:P30101,AAB37397
Molecular Weight:56,782 Da
NCBI Full Name:Protein disulfide-isomerase A3
NCBI Synonym Full Names:protein disulfide isomerase family A, member 3
NCBI Official Symbol:PDIA3  
NCBI Official Synonym Symbols:P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n  
NCBI Protein Information:protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
UniProt Protein Name:Protein disulfide-isomerase A3
UniProt Synonym Protein Names:58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
Protein Family:Protein disulfide-isomerase
UniProt Gene Name:PDIA3  
UniProt Entry Name:PDIA3_HUMAN
Anti-PDIA3 Antibody (CAB1085)Western blot analysis of extracts of various cell lines, using PDIA3 antibody (CAB1085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PDIA3 Antibody (CAB1085)Immunohistochemistry of paraffin-embedded human stomach using PDIA3 antibody (CAB1085) at dilution of 1:100 (40x lens).
Anti-PDIA3 Antibody (CAB1085)Immunohistochemistry of paraffin-embedded mouse brain using PDIA3 antibody (CAB1085) at dilution of 1:100 (40x lens).
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