Anti-PDIA2 Antibody (CAB12789)
- SKU:
- CAB12789
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PDIA2 Antibody |
| 抗体コード: | CAB12789 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, Mouse liver, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FGLK AEAA PTLR LVNL ETTK KYAP VDGG PVTA ASIT AFCH AVLN GQVK PYLL SQEI PPDW DQRP VKTL VGKN FEQV AFDE TKNV FVKF YAPW CTHC KEMA PAWE ALAE KYQD HEDI IIAE LDAT ANEL DAFA VHGF PTLK YFPA GPGR KVIE YKST RDLE TFSK FLDN GGVL PTEE PPEE PAAP FPEP PANS TMGS KEEL |
| 遺伝子ID: | 64714 |
| Uniprot: | Q13087 |
| セルラーロケーション: | Endoplasmic reticulum lumen |
| 計算された分子量: | 57kDa/58kDa |
| 観察された分子量: | 58kDa |
| 同義語: | PDIA2, PDA2, PDI, PDIP, PDIR |
| バックグラウンド: | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. |
| UniProt Protein Function: | PDIA2: Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; Isomerase; EC 5.3.4.1 Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: endoplasmic reticulum Molecular Function:disulfide oxidoreductase activity; peptidyl-proline 4-dioxygenase activity; protein binding Biological Process: protein folding; protein retention in ER |
| NCBI Summary: | Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q13087 |
| NCBI GenInfo Identifier: | 21264492 |
| NCBI Gene ID: | 64714 |
| NCBI Accession: | Q13087.2 |
| UniProt Secondary Accession: | Q13087,Q2WGM4, Q4TT67, Q6B010, Q96KJ6, Q9BW95, A6ZJ64 B4DI27, |
| UniProt Related Accession: | Q13087 |
| Molecular Weight: | 57,850 Da |
| NCBI Full Name: | Protein disulfide-isomerase A2 |
| NCBI Synonym Full Names: | protein disulfide isomerase family A member 2 |
| NCBI Official Symbol: | PDIA2 |
| NCBI Official Synonym Symbols: | PDI; PDA2; PDIP; PDIR |
| NCBI Protein Information: | protein disulfide-isomerase A2 |
| UniProt Protein Name: | Protein disulfide-isomerase A2 |
| UniProt Synonym Protein Names: | Pancreas-specific protein disulfide isomerase; PDIp |
| Protein Family: | Protein disulfide-isomerase |
| UniProt Gene Name: | PDIA2 |
| UniProt Entry Name: | PDIA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDIA2 antibody (CAB12789) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using PDIA2 antibody (CAB12789) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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