Anti-PDE6A Antibody (CAB7915)
- SKU:
- CAB7915
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-PDE6A Antibody |
| 抗体コード: | CAB7915 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 656-860 of human PDE6A (NP_000431.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 656-860 of human PDE6A (NP_000431.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EHAI HMMD IAII ATDL ALYF KKRT MFQK IVDQ SKTY ESEQ EWTQ YMML EQTR KEIV MAMM MTAC DLSA ITKP WEVQ SQVA LLVA AEFW EQGD LERT VLQQ NPIP MMDR NKAD ELPK LQVG FIDF VCTF VYKE FSRF HEEI TPML DGIT NNRK EWKA LADE YDAK MKVQ EEKK QKQQ SAKS AAAG NQPG GNPS PGGA TTSK SCCI Q |
| 遺伝子ID: | 5145 |
| Uniprot: | P16499 |
| セルラーロケーション: | Cell membrane, Cytoplasmic side, Lipid-anchor |
| 計算された分子量: | 99kDa |
| 観察された分子量: | 120kDa |
| 同義語: | PDE6A, CGPR-A, PDEA, RP43 |
| バックグラウンド: | This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. |
| UniProt Protein Function: | PDE6A: This protein participates in processes of transmission and amplification of the visual signal. Defects in PDE6A are the cause of retinitis pigmentosa type 43 (RP43). RP43 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the cyclic nucleotide phosphodiesterase family. |
| UniProt Protein Details: | Protein type:EC 3.1.4.35; Phosphodiesterase Chromosomal Location of Human Ortholog: 5q31.2-q34 Cellular Component: plasma membrane Molecular Function:3',5'-cyclic-GMP phosphodiesterase activity Biological Process: cytosolic calcium ion homeostasis; GMP metabolic process; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; Wnt receptor signaling pathway, calcium modulating pathway Disease: Retinitis Pigmentosa 43 |
| NCBI Summary: | This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P16499 |
| NCBI GenInfo Identifier: | 215274230 |
| NCBI Gene ID: | 5145 |
| NCBI Accession: | P16499.4 |
| UniProt Secondary Accession: | P16499,Q0P638, |
| UniProt Related Accession: | P16499 |
| Molecular Weight: | 99,547 Da |
| NCBI Full Name: | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha |
| NCBI Synonym Full Names: | phosphodiesterase 6A |
| NCBI Official Symbol: | PDE6A |
| NCBI Official Synonym Symbols: | PDEA; RP43; CGPR-A |
| NCBI Protein Information: | rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha |
| UniProt Protein Name: | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha |
| UniProt Synonym Protein Names: | PDE V-B1 |
| Protein Family: | Rod cGMP-specific 3',5'-cyclic phosphodiesterase |
| UniProt Gene Name: | PDE6A |
| UniProt Entry Name: | PDE6A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDE6A antibody (CAB7915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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