Anti-PDE5A Antibody (CAB6831)
- SKU:
- CAB6831
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PDE5A Antibody |
| 抗体コード: | CAB6831 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE5A (NP_001074.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HT-1080, SKOV3, A-549, 22Rv1, Mouse brain, Mouse skeletal muscle, Rat skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE5A (NP_001074.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MERA GPSF GQQR QQQQ PQQQ KQQQ RDQD SVEA WLDD HWDF TFSY FVRK ATRE MVNA WFAE RVHT IPVC KEGI RGHT ESCS CPLQ QSPR ADNS APGT PTRK ISAS EFDR PLRP IVVK DSEG TVSF LSDS EKKE QMPL TPPR FDHD EGDQ CS |
| 遺伝子ID: | 8654 |
| Uniprot: | O76074 |
| セルラーロケーション: | |
| 計算された分子量: | 94kDa/99kDa |
| 観察された分子量: | 100kDa |
| 同義語: | PDE5A, CGB-PDE, CN5A, PDE5 |
| バックグラウンド: | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms. |
| UniProt Protein Details: | Protein type:Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.35 Chromosomal Location of Human Ortholog: 4q27 Cellular Component: cytosol Molecular Function:3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP binding; metal ion binding Biological Process: cGMP catabolic process; negative regulation of T cell proliferation; positive regulation of MAP kinase activity; regulation of cGMP metabolic process; signal transduction |
| NCBI Summary: | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O76074 |
| NCBI GenInfo Identifier: | 317373261 |
| NCBI Gene ID: | 8654 |
| NCBI Accession: | O76074.2 |
| UniProt Secondary Accession: | O76074,O75026, O75887, Q86UI0, Q86V66, Q9Y6Z6, A0AV69 A8K2C4, |
| UniProt Related Accession: | O76074 |
| Molecular Weight: | 94,805 Da |
| NCBI Full Name: | cGMP-specific 3',5'-cyclic phosphodiesterase |
| NCBI Synonym Full Names: | phosphodiesterase 5A |
| NCBI Official Symbol: | PDE5A |
| NCBI Official Synonym Symbols: | CN5A; PDE5; CGB-PDE |
| NCBI Protein Information: | cGMP-specific 3',5'-cyclic phosphodiesterase |
| UniProt Protein Name: | cGMP-specific 3',5'-cyclic phosphodiesterase |
| UniProt Synonym Protein Names: | cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE |
| UniProt Gene Name: | PDE5A |
| UniProt Entry Name: | PDE5A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDE5A antibody (CAB6831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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