Anti-PDE1A Antibody (CAB10457)
- SKU:
- CAB10457
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PDE1A Antibody |
| 抗体コード: | CAB10457 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 446-545 of human PDE1A (NP_005010.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse heart, Mouse brain, Mouse kidney, Rat heart, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 446-545 of human PDE1A (NP_005010.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EASK AETS SYVA SSST TIVG LHIA DALR RSNT KGSM SDGS YSPD YSLA AVDL KSFK NNLV DIIQ QNKE RWKE LAAQ GESD LHKN SEDL VNAE EKHD ETHS |
| 遺伝子ID: | 5136 |
| Uniprot: | P54750 |
| セルラーロケーション: | |
| 計算された分子量: | 57kDa/58kDa/59kDa/60kDa/61kDa/62kDa/63kDa |
| 観察された分子量: | 55kDa |
| 同義語: | PDE1A, CAM-PDE-1A, HCAM-1, HCAM1, HSPDE1A |
| バックグラウンド: |
| UniProt Protein Function: | PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.1.4.17; Phosphodiesterase; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 2q32.1 Cellular Component: cell soma; nucleus; cytosol Molecular Function:calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding; cGMP binding Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; regulation of smooth muscle cell proliferation; nerve growth factor receptor signaling pathway; phospholipase C activation; cAMP catabolic process; innate immune response; cGMP catabolic process; signal transduction; blood coagulation |
| NCBI Summary: | Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009] |
| UniProt Code: | P54750 |
| NCBI GenInfo Identifier: | 1705942 |
| NCBI Gene ID: | 5136 |
| NCBI Accession: | P54750.2 |
| UniProt Secondary Accession: | P54750,Q86VZ0, Q9C0K8, Q9C0K9, Q9C0L0, Q9C0L1, Q9C0L2 Q9C0L3, Q9C0L4, Q9UFX3, D3DPG5, |
| UniProt Related Accession: | P54750 |
| Molecular Weight: | 535 |
| NCBI Full Name: | Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A |
| NCBI Synonym Full Names: | phosphodiesterase 1A, calmodulin-dependent |
| NCBI Official Symbol: | PDE1A |
| NCBI Official Synonym Symbols: | HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A |
| NCBI Protein Information: | calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; cam-PDE 1A; 61 kDa Cam-PDE; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase |
| UniProt Protein Name: | Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A |
| UniProt Synonym Protein Names: | 61 kDa Cam-PDE; hCam-1 |
| Protein Family: | Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase |
| UniProt Gene Name: | PDE1A |
| UniProt Entry Name: | PDE1A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDE1A antibody (CAB10457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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