Anti-PCCB Antibody (CAB5415)
- SKU:
- CAB5415
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PCCB Antibody |
| 抗体コード: | CAB5415 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-539 of human PCCB (NP_000523.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HT-29, Mouse liver, Mouse kidney, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-539 of human PCCB (NP_000523.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AFEN DVDA LCNL RDFF NYLP LSSQ DPAP VREC HDPS DRLV PELD TIVP LEST KAYN MVDI IHSV VDER EFFE IMPN YAKN IIVG FARM NGRT VGIV GNQP KVAS GCLD INSS VKGA RFVR FCDA FNIP LITF VDVP GFLP GTAQ EYGG IIRH GAKL LYAF AEAT VPKV TVIT RKAY GGAY DVMS SKHL CGDT NYAW PTAE IAVM GAKG AVEI IFKG HENV EAAQ AEYI EKFA NPFP AAVR GFVD DIIQ PSST RARI CCDL DVLA SKKV QRPW RKHA NIPL |
| 遺伝子ID: | 5096 |
| Uniprot: | P05166 |
| セルラーロケーション: | Mitochondrion matrix |
| 計算された分子量: | 58kDa/60kDa |
| 観察された分子量: | 54kDa |
| 同義語: | PCCB |
| バックグラウンド: | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PCCB: Defects in PCCB are the cause of propionic acidemia type II (PA-2). PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. Belongs to the AccD/PCCB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.4.1.3; Ligase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial Chromosomal Location of Human Ortholog: 3q21-q22 Cellular Component: mitochondrion; mitochondrial matrix; cytosol Molecular Function:propionyl-CoA carboxylase activity; ATP binding Biological Process: vitamin metabolic process; fatty acid beta-oxidation; short-chain fatty acid catabolic process; cellular lipid metabolic process; biotin metabolic process; water-soluble vitamin metabolic process Disease: Propionic Acidemia |
| NCBI Summary: | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
| UniProt Code: | P05166 |
| NCBI GenInfo Identifier: | 124106304 |
| NCBI Gene ID: | 5096 |
| NCBI Accession: | P05166.3 |
| UniProt Secondary Accession: | P05166,Q16813, Q96CX0, B7Z2Z4, |
| UniProt Related Accession: | P05166 |
| Molecular Weight: | 60,521 Da |
| NCBI Full Name: | Propionyl-CoA carboxylase beta chain, mitochondrial |
| NCBI Synonym Full Names: | propionyl CoA carboxylase, beta polypeptide |
| NCBI Official Symbol: | PCCB |
| NCBI Protein Information: | propionyl-CoA carboxylase beta chain, mitochondrial; PCCase subunit beta; propanoyl-CoA:carbon dioxide ligase subunit beta; propionyl Coenzyme A carboxylase, beta polypeptide |
| UniProt Protein Name: | Propionyl-CoA carboxylase beta chain, mitochondrial |
| UniProt Synonym Protein Names: | Propanoyl-CoA:carbon dioxide ligase subunit beta |
| Protein Family: | Phycoerythrocyanin |
| UniProt Gene Name: | PCCB |
| UniProt Entry Name: | PCCB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PCCB antibody (CAB5415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunofluorescence analysis of U2OS cells using PCCB antibody (CAB5415). Blue: DAPI for nuclear staining. |
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