Anti-PARN Antibody (CAB4002)
- SKU:
- CAB4002
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
抗体名: | Anti-PARN Antibody |
抗体コード: | CAB4002 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC IF |
反応性: | Human |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PARN (NP_002573.1). |
申し込み: | WB IHC IF |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
反応性: | Human |
ポジティブサンプル: | MCF7, HepG2 |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PARN (NP_002573.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | MEII RSNF KSNL HKVY QAIE EADF FAID GEFS GISD GPSV SALT NGFD TPEE RYQK LKKH SMDF LLFQ FGLC TFKY DYTD SKYI TKSF NFYV FPKP FNRS SPDV KFVC QSSS IDFL ASQG FDFN KVFR NGIP YLNQ EEER QLRE QYDE KRSQ ANGA GALS YVSP NTSK CPVT IPED QKKF IDQV VEKI EDLL QSEE NKNL DLEP CTGF QRKL IYQT LSWK YPKG IHVE TLET EKKE RYIV ISKV DEEE RKRR EQQK HAKE QEEL NDAV GFSR VIHA IANS |
遺伝子ID: | 5073 |
Uniprot: | O95453 |
セルラーロケーション: | Cytoplasm, Nucleus, nucleolus |
計算された分子量: | 52kDa/66kDa/67kDa/73kDa |
観察された分子量: | 73kDa |
同義語: | PARN, DAN, DKCB6, PFBMFT4 |
バックグラウンド: | The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
UniProt Protein Function: | PARN: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Ubiquitous. Belongs to the CAF1 family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Ribonuclease; RNA-binding; EC 3.1.13.4; Nucleolus Chromosomal Location of Human Ortholog: 16p13 Cellular Component: cytoplasm; nucleolus; cytosol; nucleus Molecular Function:protein binding; poly(A)-specific ribonuclease activity; nuclease activity; mRNA 3'-UTR binding; metal ion binding; nucleotide binding; protein kinase binding Biological Process: poly(A) tail shortening; RNA modification; mRNA catabolic process, nonsense-mediated decay; female gamete generation; gene expression; mRNA catabolic process, deadenylation-dependent decay Disease: Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 4; Dyskeratosis Congenita, Autosomal Recessive, 6 |
NCBI Summary: | The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
UniProt Code: | O95453 |
NCBI GenInfo Identifier: | 60390262 |
NCBI Gene ID: | 5073 |
NCBI Accession: | O95453.1 |
UniProt Related Accession: | O95453 |
Molecular Weight: | ~ 73kDa |
NCBI Full Name: | Poly(A)-specific ribonuclease PARN |
NCBI Synonym Full Names: | poly(A)-specific ribonuclease |
NCBI Official Symbol: | PARN |
NCBI Official Synonym Symbols: | DAN; DKCB6; PFBMFT4 |
NCBI Protein Information: | poly(A)-specific ribonuclease PARN |
UniProt Protein Name: | Poly(A)-specific ribonuclease PARN |
UniProt Synonym Protein Names: | Deadenylating nuclease; Deadenylation nuclease; Polyadenylate-specific ribonuclease |
Protein Family: | Poly(A)-specific ribonuclease |
UniProt Gene Name: | PARN |
UniProt Entry Name: | PARN_HUMAN |
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