Anti-PARL Antibody (CAB8232)
- SKU:
- CAB8232
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | PARL Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8232 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human |
| ポジティブサンプル: | HepG2, MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAWR GWAQ RGWG CGQA WGAS VGGR SCEE LTAV LTPP QLLG RRFN FFIQ QKCG FRKA PRKV EPRR SDPG TSGE AYKR SALI PPVE ETVF YPSP YPIR SLIK PLFF TVGF TGCA FGSA AIWQ YESL KSRV QSYF DGIK ADWL DSIR PQKE GDFR KEIN KWWN NLSD GQRT VT |
| 遺伝子ID: | 55486 |
| Uniprot: | Q9H300 |
| セルラーロケーション: | Mitochondrion inner membrane, Multi-pass membrane protein, Nucleus |
| 計算された分子量: | 36kDa/42kDa |
| 観察された分子量: | 49kDa |
| 同義語: | PARL, PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1 |
| バックグラウンド: | This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease. |
| UniProt Protein Function: | PSARL: a mitochondrial intramembrane protease that is associated with insulin resistance and type 2 diabetes. Induced in humans by caloric restriction. Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. Induced in humans by caloric restriction. Interacts with PSEN1 and PSEN2. Binds OPA1. Two isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Membrane protein, integral; Protease; EC 3.4.21.105; Membrane protein, multi-pass; Mitochondrial Chromosomal Location of Human Ortholog: 3q27.1 Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus Molecular Function:protein binding; serine-type endopeptidase activity; endopeptidase activity Biological Process: membrane protein proteolysis; proteolysis |
| NCBI Summary: | This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H300 |
| NCBI GenInfo Identifier: | 143811433 |
| NCBI Gene ID: | 55486 |
| NCBI Accession: | Q9H300.2 |
| UniProt Secondary Accession: | Q9H300,Q96CQ4, Q9BTJ6, Q9P1E3, |
| UniProt Related Accession: | Q9H300 |
| Molecular Weight: | 36,589 Da |
| NCBI Full Name: | Presenilins-associated rhomboid-like protein, mitochondrial |
| NCBI Synonym Full Names: | presenilin associated, rhomboid-like |
| NCBI Official Symbol: | PARL |
| NCBI Official Synonym Symbols: | PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2 |
| NCBI Protein Information: | presenilins-associated rhomboid-like protein, mitochondrial; rhomboid 7 homolog 1; mitochondrial intramembrane cleaving protease PARL; mitochondrial intramembrane-cleaving protease PARL |
| UniProt Protein Name: | Presenilins-associated rhomboid-like protein, mitochondrial |
| UniProt Synonym Protein Names: | Mitochondrial intramembrane cleaving protease PARLP-beta; Pbeta |
| Protein Family: | Presenilins-associated rhomboid-like protein |
| UniProt Gene Name: | PARL |
| UniProt Entry Name: | PARL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PARL antibody (CAB8232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using PARL antibody (CAB8232) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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