Anti-PABPC4 抗体 (CAB5948)

抗体名:	Polyclonal AntibodyPC4 Rabbit Polyclonal Antibody
抗体コード:	CAB5948
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	HepG2, Jurkat, Mouse spleen, Mouse heart, Rat brain, Rat heart

免疫原:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	VTEM NGRI VGSK PLYV ALAQ RKEE RKAH LTNQ YMQR VAGM RALP ANAI LNQF QPAA GGYF VPAV PQAQ GRPP YYTP NQLA QMRP NPRW QQGG RPQG FQGM P
遺伝子ID:	8761
Uniprot:	Q13310
セルラーロケーション:	Cytoplasm
計算された分子量:	69kDa/70kDa/72kDa
観察された分子量:	79kDa

同義語:

PABPC4, APP-1, APP1, PABP4, iPABP

バックグラウンド:

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PABP 4: poly(A) binding protein 4 may be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated. Localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells.
UniProt Protein Details:	Protein type:RNA-binding; Translation Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: cytoplasm; nucleus; ribonucleoprotein complex; stress granule Molecular Function:poly(A) binding; poly(rC) binding; poly(U) binding; protein binding Biological Process: blood coagulation; RNA catabolic process; RNA processing; translation
NCBI Summary:	Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	Q13310
NCBI GenInfo Identifier:	12229875
NCBI Gene ID:	8761
NCBI Accession:	Q13310.1
UniProt Secondary Accession:	Q13310,Q4VC03, Q6P0N3, B1ANQ8,
UniProt Related Accession:	Q13310
Molecular Weight:	72,391 Da
NCBI Full Name:	Polyadenylate-binding protein 4
NCBI Synonym Full Names:	poly(A) binding protein cytoplasmic 4
NCBI Official Symbol:	PABPC4
NCBI Official Synonym Symbols:	APP1; APP-1; PABP4; iPABP
NCBI Protein Information:	polyadenylate-binding protein 4
UniProt Protein Name:	Polyadenylate-binding protein 4
UniProt Synonym Protein Names:	Activated-platelet protein 1; APP-1; Inducible poly(A)-binding protein; iPABP
UniProt Gene Name:	PABPC4
UniProt Entry Name:	PABP4_HUMAN

	Western blot analysis of extracts of various cell lines, using PABPC4 antibody (CAB5948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human esophagus using PABPC4 antibody (CAB5948) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PABPC4 antibody (CAB5948) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PABPC4 antibody (CAB5948) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

PABPC4 Antibody (PACO11046)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)