Anti-P4HA2 Antibody (CAB4262)
- SKU:
- CAB4262
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-P4HA2 Antibody |
| 抗体コード: | CAB4262 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-533 of human P4HA2 (NP_001136070.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SKOV3, Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-533 of human P4HA2 (NP_001136070.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LSLD PSHE RAGG NLRY FEQL LEEE REKT LTNQ TEAE LATP EGIY ERPV DYLP ERDV YESL CRGE GVKL TPRR QKRL FCRY HHGN RAPQ LLIA PFKE EDEW DSPH IVRY YDVM SDEE IERI KEIA KPKL ARAT VRDP KTGV LTVA SYRV SKSS WLEE DDDP VVAR VNRR MQHI TGLT VKTA ELLQ VANY GVGG QYEP HFDF SRRP FDSG LKTE GNRL ATFL NYMS DVEA GGAT VFPD LGAA IWPK KGTA VFWY NLLR SGEG DYRT RHAA CPVL VGCK WVSN KWFH ERGQ EFLR PCGS TEVD |
| 遺伝子ID: | 8974 |
| Uniprot: | O15460 |
| セルラーロケーション: | Endoplasmic reticulum lumen |
| 計算された分子量: | 60kDa |
| 観察された分子量: | 65kDa |
| 同義語: | P4HA2, MYP25 |
| バックグラウンド: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | P4HA2: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.11.2 Chromosomal Location of Human Ortholog: 5q31 Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum lumen; endoplasmic reticulum; cytoplasm; nucleus Molecular Function:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; procollagen-proline 4-dioxygenase activity; electron carrier activity; L-ascorbic acid binding; iron ion binding Biological Process: peptidyl-proline hydroxylation to 4-hydroxy-L-proline |
| NCBI Summary: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15460 |
| NCBI GenInfo Identifier: | 20455169 |
| NCBI Gene ID: | 8974 |
| NCBI Accession: | O15460.1 |
| UniProt Secondary Accession: | O15460,Q8WWN0, D3DQ85, D3DQ86, |
| UniProt Related Accession: | O15460 |
| Molecular Weight: | 535 |
| NCBI Full Name: | Prolyl 4-hydroxylase subunit alpha-2 |
| NCBI Synonym Full Names: | prolyl 4-hydroxylase, alpha polypeptide II |
| NCBI Official Symbol: | P4HA2 |
| NCBI Protein Information: | prolyl 4-hydroxylase subunit alpha-2; 4-PH alpha 2; 4-PH alpha-2; C-P4Halpha(II); collagen prolyl 4-hydroxylase alpha(II); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II |
| UniProt Protein Name: | Prolyl 4-hydroxylase subunit alpha-2 |
| UniProt Synonym Protein Names: | Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 |
| Protein Family: | Prolyl 4-hydroxylase |
| UniProt Gene Name: | P4HA2 |
| UniProt Entry Name: | P4HA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using P4HA2 antibody (CAB4262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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