Anti-P4HA1 Antibody (CAB10538)
- SKU:
- CAB10538
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-P4HA1 Antibody |
| 抗体コード: | CAB10538 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 235-534 of human P4HA1 (NP_001017962.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-87MG, LO2, A-431, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 235-534 of human P4HA1 (NP_001017962.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DPEH QRAN GNLK YFEY IMAK EKDV NKSA SDDQ SDQK TTPK KKGV AVDY LPER QKYE MLCR GEGI KMTP RRQK KLFC RYHD GNRN PKFI LAPA KQED EWDK PRII RFHD IISD AEIE IVKD LAKP RLRR ATIS NPIT GDLE TVHY RISK SAWL SGYE NPVV SRIN MRIQ DLTG LDVS TAEE LQVA NYGV GGQY EPHF DFAR KDEP DAFK ELGT GNRI ATWL FYMS DVSA GGAT VFPE VGAS VWPK KGTA VFWY NLFA SGEG DYST RHAA CPVL VGNK WVSN KWLH ERGQ EFRR PCTL SELE |
| 遺伝子ID: | 5033 |
| Uniprot: | P13674 |
| セルラーロケーション: | Endoplasmic reticulum lumen |
| 計算された分子量: | 58kDa/60kDa/61kDa |
| 観察された分子量: | 61kDa |
| 同義語: | P4HA1, P4HA |
| バックグラウンド: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.2; Endoplasmic reticulum; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen Molecular Function:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline |
| NCBI Summary: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13674 |
| NCBI GenInfo Identifier: | 2507090 |
| NCBI Gene ID: | 5033 |
| NCBI Accession: | P13674.2 |
| UniProt Secondary Accession: | P13674,Q15082, Q15083, Q5VSQ5, C9JL12, |
| UniProt Related Accession: | P13674 |
| Molecular Weight: | 534 |
| NCBI Full Name: | Prolyl 4-hydroxylase subunit alpha-1 |
| NCBI Synonym Full Names: | prolyl 4-hydroxylase, alpha polypeptide I |
| NCBI Official Symbol: | P4HA1 |
| NCBI Official Synonym Symbols: | P4HA |
| NCBI Protein Information: | prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I |
| UniProt Protein Name: | Prolyl 4-hydroxylase subunit alpha-1 |
| UniProt Synonym Protein Names: | Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1 |
| Protein Family: | Prolyl 4-hydroxylase |
| UniProt Gene Name: | P4HA1 |
| UniProt Entry Name: | P4HA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using P4HA1 antibody (CAB10538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 8s. |
 | Immunofluorescence analysis of L929 cells using P4HA1 Rabbit pAb (CAB10538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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