Anti-OTUB1 Antibody (CAB10313)
- SKU:
- CAB10313
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-OTUB1 Antibody |
| 抗体コード: | CAB10313 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human OTUB1 |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, Mouse intestine, Mouse liver |
| 免疫原: | A synthetic peptide of human OTUB1 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 55611 |
| Uniprot: | Q96FW1 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 31kDa/35kDa |
| 観察された分子量: | 31-35kDa |
| 同義語: | OTUB1, HSPC263, OTB1, OTU1 |
| バックグラウンド: | The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: cytoplasm; nucleus Molecular Function:NEDD8-specific protease activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity Biological Process: adaptive immune response; DNA repair; response to DNA damage stimulus |
| NCBI Summary: | The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96FW1 |
| NCBI GenInfo Identifier: | 44888286 |
| NCBI Gene ID: | 55611 |
| NCBI Accession: | Q96FW1.2 |
| UniProt Secondary Accession: | Q96FW1,Q32Q78, Q96II3, Q9NXQ4, Q9P0B8, |
| UniProt Related Accession: | Q96FW1 |
| Molecular Weight: | 35,285 Da |
| NCBI Full Name: | Ubiquitin thioesterase OTUB1 |
| NCBI Synonym Full Names: | OTU deubiquitinase, ubiquitin aldehyde binding 1 |
| NCBI Official Symbol: | OTUB1 |
| NCBI Official Synonym Symbols: | OTB1; OTU1; HSPC263 |
| NCBI Protein Information: | ubiquitin thioesterase OTUB1 |
| UniProt Protein Name: | Ubiquitin thioesterase OTUB1 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1 |
| Protein Family: | Ubiquitin thioesterase |
| UniProt Gene Name: | OTUB1 |
| UniProt Entry Name: | OTUB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OTUB1 antibody (CAB10313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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