Anti-ORC4L Antibody (CAB7705)
- SKU:
- CAB7705
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-ORC4L Antibody |
| 抗体コード: | CAB7705 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human ORC4L (NP_002543.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, MCF7, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human ORC4L (NP_002543.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IAVI GLTC RLDI LELL EKRV KSRF SHRQ IHLM NSFG FPQY VKIF KEQL SLPA EFPD KVFA EKWN ENVQ YLSE DRSV QEVL QKHF NISK NLRS LHML LMLA LNRV TASH PFMT AVDL MEAS QLCS MDSK ANIV HGLS VLEI CLII AMKH LNDI YEEE PFNF QMVY NEFQ KFVQ RKAH SVYN FEKP VVMK AFEH LQQL ELIK PMER TSGN SQRE YQLM KLLL DNTQ IMNA LQKY PNCP TDVR QWAT SSLS WL |
| 遺伝子ID: | 5000 |
| Uniprot: | O43929 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 40kDa/42kDa/50kDa |
| 観察された分子量: | 50kDa |
| 同義語: | ORC4, ORC4L, ORC4P |
| バックグラウンド: | The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. |
| UniProt Protein Function: | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3. |
| NCBI Summary: | The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010] |
| UniProt Code: | O43929 |
| NCBI GenInfo Identifier: | 300116159 |
| NCBI Gene ID: | 5000 |
| NCBI Accession: | NP_001177808.1 |
| UniProt Secondary Accession: | O43929,Q96C42, B7Z3D0, B7Z5F1, D3DP86, F5H069, |
| UniProt Related Accession: | O43929 |
| Molecular Weight: | 47 |
| NCBI Full Name: | origin recognition complex subunit 4 isoform 1 |
| NCBI Synonym Full Names: | origin recognition complex subunit 4 |
| NCBI Official Symbol: | ORC4 |
| NCBI Official Synonym Symbols: | ORC4L; ORC4P |
| NCBI Protein Information: | origin recognition complex subunit 4 |
| UniProt Protein Name: | Origin recognition complex subunit 4 |
| Protein Family: | Origin recognition complex |
| UniProt Gene Name: | ORC4 |
 | Western blot analysis of extracts of various cell lines, using ORC4 antibody (CAB7705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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