Anti-NUP98 Antibody (CAB0530)
- SKU:
- CAB0530
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-NUP98 Antibody |
| 抗体コード: | CAB0530 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 621-920 of human NUP98 (NP_624357.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-549, LO2, HeLa, Jurkat, HL-60 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 621-920 of human NUP98 (NP_624357.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SKPV DENH QQDG DEDS LVSH FYTN PIAK PIPQ TPES AGNK HSNS NSVD DTIV ALNM RAAL RNGL EGSS EETS FHDE SLQD DREE IENN SYHM HPAG IILT KVGY YTIP SMDD LAKI TNEK GECI VSDF TIGR KGYG SIYF EGDV NLTN LNLD DIVH IRRK EVVV YLDD NQKP PVGE GLNR KAEV TLDG VWPT DKTS RCLI KSPD RLAD INYE GRLE AVSR KQGA QFKE YRPE TGSW VFKV SHFS KYGL QDSD EEEE EHPS KTST KKLK TAPL PPAS QTTP LQMA LNGK PAPP PQVE KKGQ |
| 遺伝子ID: | 4928 |
| Uniprot: | P52948 |
| セルラーロケーション: | Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex |
| 計算された分子量: | 96kDa/97kDa/186kDa/187kDa/195kDa/197kDa |
| 観察された分子量: | 105kDa |
| 同義語: | NUP98, ADIR2, NUP196, NUP96 |
| バックグラウンド: | Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. |
| UniProt Protein Function: | NUP98: Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. May interact with RAE1. Interacts with vesicular stomatitis virus protein M. Belongs to the nucleoporin GLFG family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Nucleoporin Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: nucleoplasm; kinetochore; nuclear membrane; nuclear pore; nuclear envelope; cytosol; nuclear inclusion body Molecular Function:protein binding; structural constituent of nuclear pore; transporter activity; peptide binding Biological Process: nuclear pore complex assembly; mRNA transport; viral reproduction; protein import into nucleus, docking; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; nucleocytoplasmic transport; pathogenesis; viral infectious cycle; glucose transport; nuclear pore organization and biogenesis; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; DNA replication; transmembrane transport |
| NCBI Summary: | Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, May 2010] |
| UniProt Code: | P52948 |
| NCBI GenInfo Identifier: | 308153660 |
| NCBI Gene ID: | 4928 |
| NCBI Accession: | P52948.4 |
| UniProt Secondary Accession: | P52948,Q8IUT2, Q8WYB0, Q96E54, Q9H3Q4, Q9NT02, Q9UF57 Q9UHX0, Q9Y6J4, Q9Y6J5, |
| UniProt Related Accession: | P52948 |
| Molecular Weight: | 1817 |
| NCBI Full Name: | Nuclear pore complex protein Nup98-Nup96 |
| NCBI Synonym Full Names: | nucleoporin 98kDa |
| NCBI Official Symbol: | NUP98 |
| NCBI Official Synonym Symbols: | ADIR2; NUP96; NUP196 |
| NCBI Protein Information: | nuclear pore complex protein Nup98-Nup96; nucleoporin 98kD; GLFG-repeat containing nucleoporin |
| UniProt Protein Name: | Nuclear pore complex protein Nup98-Nup96 |
| UniProt Synonym Protein Names: | 98 kDa nucleoporin; Nucleoporin Nup98; Nup98 |
| UniProt Gene Name: | NUP98 |
| UniProt Entry Name: | NUP98_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NUP98 antibody (CAB0530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of L929 cells using NUP98 antibody (CAB0530) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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