Anti-NUP85 Antibody (CAB3504)
- SKU:
- CAB3504
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-NUP85 Antibody |
| 抗体コード: | CAB3504 |
| 抗体サイズ: | 100µL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human NUP85 |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse lung, Mouse thymus |
| 免疫原: | Recombinant protein of human NUP85 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 79902 |
| Uniprot: | Q9BW27 |
| セルラーロケーション: | Chromosome, Cytoplasm, Nucleus, Nucleus membrane, centromere, cytoskeleton, kinetochore, nuclear pore complex, spindle |
| 計算された分子量: | 52kDa/69kDa/75kDa |
| 観察された分子量: | 70kDa |
| 同義語: | NUP85, FROUNT, Nup75 |
| バックグラウンド: | This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NUP85: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. Belongs to the nucleoporin Nup85 family. |
| UniProt Protein Details: | Protein type:Nucleoporin Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: cytosol; kinetochore; membrane; nuclear envelope Molecular Function:protein binding; structural constituent of nuclear pore Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; positive regulation of transcription, DNA-dependent; protein import into nucleus; protein sumoylation; RNA-mediated gene silencing; sister chromatid cohesion; tRNA export from nucleus; viral reproduction; viral transcription |
| NCBI Summary: | This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q9BW27 |
| NCBI GenInfo Identifier: | 74733394 |
| NCBI Gene ID: | 79902 |
| NCBI Accession: | Q9BW27.1 |
| UniProt Secondary Accession: | Q9BW27,Q8NDI4, Q9H9U1, B4DMQ3, B4DPW1, |
| UniProt Related Accession: | Q9BW27 |
| Molecular Weight: | 69,800 Da |
| NCBI Full Name: | Nuclear pore complex protein Nup85 |
| NCBI Synonym Full Names: | nucleoporin 85 |
| NCBI Official Symbol: | NUP85 |
| NCBI Official Synonym Symbols: | Nup75; FROUNT |
| NCBI Protein Information: | nuclear pore complex protein Nup85 |
| UniProt Protein Name: | Nuclear pore complex protein Nup85 |
| UniProt Synonym Protein Names: | 85 kDa nucleoporin; FROUNT; Nucleoporin Nup75; Nucleoporin Nup85; Pericentrin-1 |
| Protein Family: | Nucleoporin |
| UniProt Gene Name: | NUP85 |
| UniProt Entry Name: | NUP85_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NUP85 antibody (CAB3504). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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