Anti-NUP153 Antibody (RACO0430)
- SKU:
- RACO0430
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | NUP153 Antibody |
| Product SKU: | RACO0430 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Nup153 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC). |
| シノニム: | Nuclear pore complex protein Nup153 (153 kDa nucleoporin) (Nucleoporin Nup153), NUP153 |
 | Immunofluorescence staining of Hela Cells with RACO0430 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | IHC image of RACO0430 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | NUP153: Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. |
| UniProt Protein Details: | Protein type:Nuclear export; Nucleoporin Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: cytoplasm; nuclear inclusion body; nuclear membrane; nuclear pore; nucleolus; nucleoplasm Molecular Function:identical protein binding; nucleocytoplasmic transporter activity; protein anchor; protein binding; structural constituent of nuclear pore Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; negative regulation of RNA export from nucleus; nuclear pore complex assembly; protein sumoylation; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription |
| NCBI Summary: | Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P49790 |
| NCBI GenInfo Identifier: | 206729891 |
| NCBI Gene ID: | 9972 |
| NCBI Accession: | P49790.2 |
| UniProt Secondary Accession: | P49790,Q4LE47, Q5T9I7, Q7Z743, B4DIK2, E7EPX5, F6QR24 |
| UniProt Related Accession: | P49790 |
| Molecular Weight: | 157,338 Da |
| NCBI Full Name: | Nuclear pore complex protein Nup153 |
| NCBI Synonym Full Names: | nucleoporin 153 |
| NCBI Official Symbol: | NUP153 |
| NCBI Official Synonym Symbols: | N153; HNUP153 |
| NCBI Protein Information: | nuclear pore complex protein Nup153 |
| UniProt Protein Name: | Nuclear pore complex protein Nup153 |
| UniProt Synonym Protein Names: | 153 kDa nucleoporin; Nucleoporin Nup153 |
| Protein Family: | Nuclear pore complex protein |
| UniProt Gene Name: | NUP153 |
| UniProt Entry Name: | NU153_HUMAN |