Anti-NTMT1 Antibody (CAB7098)
- SKU:
- CAB7098
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NTMT1 Antibody |
| 抗体コード: | CAB7098 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, SKOV3, U-87MG, A-431, Raji, Mouse brain, Rat ovary |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTSE VIED EKQF YSKA KTYW KQIP PTVD GMLG GYGH ISSI DINS SRKF LQRF LREG PNKT GTSC ALDC GAGI GRIT KRLL LPLF REVD MVDI TEDF LVQA KTYL GEEG KRVR NYFC CGLQ DFTP EPDS YDVI WIQW VIGH LTDQ HLAE FLRR CKGS LRPN GIIV IKDN MAQE GVIL DDVD SSVC RDLD VVRR IICS AGLS LLAE ERQE NLPD EIYH VYSF ALR |
| 遺伝子ID: | 28989 |
| Uniprot: | Q9BV86 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 15kDa/25kDa |
| 観察された分子量: | 25kDa |
| 同義語: | NTMT1, AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NRMT1, NTM1A |
| バックグラウンド: |
| UniProt Protein Function: | METTL11A: Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]- Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di- methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. Belongs to the methyltransferase superfamily. NTM1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.1.1.244; Methyltransferase, protein N-term; Methyltransferase Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein methyltransferase activity Biological Process: N-terminal peptidyl-proline di-methylation; spindle organization and biogenesis; N-terminal peptidyl-alanine tri-methylation; chromosome segregation |
| NCBI Summary: | The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).[supplied by OMIM, Nov 2010] |
| UniProt Code: | Q9BV86 |
| NCBI GenInfo Identifier: | 74761281 |
| NCBI Gene ID: | 28989 |
| NCBI Accession: | Q9BV86.3 |
| UniProt Secondary Accession: | Q9BV86,Q5SZB9, Q9UI28, A8K4J2, A8K8G7, |
| UniProt Related Accession: | Q9BV86 |
| Molecular Weight: | 15,896 Da |
| NCBI Full Name: | N-terminal Xaa-Pro-Lys N-methyltransferase 1 |
| NCBI Synonym Full Names: | N-terminal Xaa-Pro-Lys N-methyltransferase 1 |
| NCBI Official Symbol: | NTMT1 |
| NCBI Official Synonym Symbols: | NRMT; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A |
| NCBI Protein Information: | N-terminal Xaa-Pro-Lys N-methyltransferase 1; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A; alpha N-terminal protein methyltransferase 1A; methyltransferase-like protein 11A |
| UniProt Protein Name: | N-terminal Xaa-Pro-Lys N-methyltransferase 1 |
| UniProt Synonym Protein Names: | Alpha N-terminal protein methyltransferase 1A; Methyltransferase-like protein 11A; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A; NTM1A |
| Protein Family: | N-terminal Xaa-Pro-Lys N-methyltransferase |
| UniProt Gene Name: | NTMT1 |
| UniProt Entry Name: | NTM1A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NTMT1 antibody (CAB7098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of A-549 cells using NTMT1 antibody (CAB7098). Blue: DAPI for nuclear staining. |
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