Anti-NSUN4 Antibody (CAB14983)
- SKU:
- CAB14983
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NSUN4 Antibody |
| 抗体コード: | CAB14983 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-290 of human NSUN4 (NP_950245.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y, HT-29, HeLa, HepG2, Mouse brain, Mouse testis, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-290 of human NSUN4 (NP_950245.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RYKK KWAA TEPK FPAV RLAL QNFD MTYS VQFG DLWP SIRV SLLS EQKY GALV NNFA AWDH VSAK LEQL SAKD FVNE AISH WELQ SEGG QSAA PSPA SWAC SPNL RCFT FDRG DISR FPPA RPGS LGVM EYYL MDAA SLLP VLAL GLQP GDIV LDLC AAPG GKTL ALLQ TGCC RNLA ANDL SPSR IARL QKIL HSYV PEEI RDGN QVRV TSWD GRKW GELE GDTY DRVL VDVP CTTD RHSL HEEE NNIF KRSR KKER QILP VLQV Q |
| 遺伝子ID: | 387338 |
| Uniprot: | Q96CB9 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 16kDa/21kDa/37kDa/43kDa |
| 観察された分子量: | 43kDa |
| 同義語: | NSUN4, SHTAP |
| バックグラウンド: |
| UniProt Protein Function: | Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4. |
| UniProt Code: | Q96CB9 |
| NCBI GenInfo Identifier: | 371123153 |
| NCBI Gene ID: | 387338 |
| NCBI Accession: | NP_001243056.1 |
| UniProt Secondary Accession: | Q96CB9,Q5TDF7, Q96AN8, Q9HAJ8, A8K6S6, B3KQ50, B4DHA4 |
| UniProt Related Accession: | Q96CB9 |
| Molecular Weight: | 43 kDa |
| NCBI Full Name: | 5-methylcytosine rRNA methyltransferase NSUN4 isoform b |
| NCBI Synonym Full Names: | NOP2/Sun RNA methyltransferase family member 4 |
| NCBI Official Symbol: | NSUN4 |
| NCBI Official Synonym Symbols: | SHTAP |
| NCBI Protein Information: | 5-methylcytosine rRNA methyltransferase NSUN4 |
| UniProt Protein Name: | 5-methylcytosine rRNA methyltransferase NSUN4 |
| UniProt Synonym Protein Names: | NOL1/NOP2/Sun domain family member 4 |
| Protein Family: | Sperm head and tail associated protein |
| UniProt Gene Name: | NSUN4 |
 | Western blot analysis of extracts of various cell lines, using NSUN4 antibody (CAB14983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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