Anti-NSF Antibody (CAB0926)
- SKU:
- CAB0926
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Zebrafish
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
抗体名: | Anti-NSF Antibody |
抗体コード: | CAB0926 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC IF IP |
反応性: | Human, Mouse, Rat, Zebrafish |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NSF (NP_006169.2). |
申し込み: | WB IHC IF IP |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200 |
反応性: | Human, Mouse, Rat, Zebrafish |
ポジティブサンプル: | Mouse brain, Rat brain |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NSF (NP_006169.2). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | MAGR SMQA ARCP TDEL SLTN CAVV NEKD FQSG QHVI VRTS PNHR YTFT LKTH PSVV PGSI AFSL PQRK WAGL SIGQ EIEV SLYT FDKA KQCI GTMT IEID FLQK KSID SNPY DTDK MAAE FIQQ FNNQ AFSV GQQL VFSF NEKL FGLL VKDI EAMD PSIL KGEP ATGK RQKI EVGL VVGN SQVA FEKA ENSS LNLI GKAK TKEN RQSI INPD WNFE KMGI GGLD KEFS DIFR RAFA SRVF PPEI VEQM GC |
遺伝子ID: | 4905 |
Uniprot: | P46459 |
セルラーロケーション: | Cytoplasm |
計算された分子量: | 71kDa/82kDa |
観察された分子量: | 82kDa |
同義語: | NSF, SEC18, SKD2 |
バックグラウンド: | Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity). |
UniProt Protein Function: | NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns. |
UniProt Protein Details: | Protein type:Autophagy; EC 3.6.4.6; Adaptor/scaffold; Protease; Vesicle Chromosomal Location of Human Ortholog: 17q21 Cellular Component: cytoplasm; cytosol; dendritic shaft; Golgi membrane; Golgi stack; lysosomal membrane; myelin sheath; plasma membrane; postsynaptic density Molecular Function:ATP binding; ATPase activity; ATPase activity, coupled; metal ion binding; PDZ domain binding; protein binding; protein complex binding; protein kinase binding; Rab GTPase binding; syntaxin-1 binding Biological Process: cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; exocytosis; Golgi to plasma membrane protein transport; Golgi vesicle docking; intra-Golgi vesicle-mediated transport; intracellular protein transport; plasma membrane fusion; positive regulation of protein catabolic process; positive regulation of receptor recycling; post-translational protein modification; potassium ion transport; protein amino acid N-linked glycosylation via asparagine; regulation of exocytosis; synaptic transmission; vesicle-mediated transport |
UniProt Code: | P46459 |
NCBI GenInfo Identifier: | 257051048 |
NCBI Gene ID: | 4905 |
NCBI Accession: | P46459.3 |
UniProt Secondary Accession: | P46459,Q8N6D7, Q9UKZ2, A8K2D9, B4DFA2, |
UniProt Related Accession: | P46459 |
Molecular Weight: | 71,584 Da |
NCBI Full Name: | Vesicle-fusing ATPase |
NCBI Synonym Full Names: | N-ethylmaleimide sensitive factor |
NCBI Official Symbol: | NSF |
NCBI Official Synonym Symbols: | SKD2 |
NCBI Protein Information: | vesicle-fusing ATPase |
UniProt Protein Name: | Vesicle-fusing ATPase |
UniProt Synonym Protein Names: | N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF |
Protein Family: | NSFL1 cofactor |
UniProt Gene Name: | NSF |
UniProt Entry Name: | NSF_HUMAN |