Anti-NOP14 Antibody (CAB10361)

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SKU:
CAB10361
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

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抗体名:Anti-NOP14 Antibody
抗体コード:CAB10361
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NOP14 (NP_001278908.1).
申し込み:WB
推奨希釈:WB 1:500 - 1:2000
反応性:Human
ポジティブサンプル:HeLa, U-87MG, HT-29, Jurkat, MCF7
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NOP14 (NP_001278908.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MAKA KKVG ARRK ASGA PAGA RGGP AKAN SNPF EVKV NRQK FQIL GRKT RHDV GLPG VSRA RALR KRTQ TLLK EYKE RDKS NVFR DKRF GEYN SNMS PEEK MMKR FALE QQRH HEKK SIYN LNED EELT HYGQ SLAD IEKH NDIV DSDS DAED RGTL SAEL TAAH FGGG GGLL HKKT QQEG EERE KPKS RKEL IEEL IAKS KQEK RERQ AQRE DALE LTEK LDQD WKEI QTLL SHKT PKSE
遺伝子ID:8602
Uniprot:P78316
セルラーロケーション:Nucleus, nucleolus
計算された分子量:91kDa/97kDa
観察された分子量:115kDa
同義語:NOP14, C4orf9, NOL14, RES4-25, RES425, UTP2
バックグラウンド:This gene encodes a protein that plays a role in pre-18s rRNA processing and small ribosomal subunit assembly. The encoded protein may be involved in the regulation of pancreatic cancer cell proliferation and migration. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:NOP14: Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm. Belongs to the NOP14 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleolus

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: membrane; mitochondrion; nucleolus; nucleoplasm; nucleus; small subunit processome

Molecular Function:enzyme binding; RNA binding; snoRNA binding

Biological Process: endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of SSU-rRNA; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ribosomal small subunit biogenesis and assembly; rRNA processing

NCBI Summary:This gene encodes a protein that plays a role in pre-18s rRNA processing and small ribosomal subunit assembly. The encoded protein may be involved in the regulation of pancreatic cancer cell proliferation and migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:P78316
NCBI GenInfo Identifier:56404449
NCBI Gene ID:8602
NCBI Accession:P78316.3
UniProt Secondary Accession:P78316,Q7LGI5, Q7Z6K0, Q8TBR6, D3DVR6,
UniProt Related Accession:P78316
Molecular Weight:98kDa
NCBI Full Name:Nucleolar protein 14
NCBI Synonym Full Names:NOP14 nucleolar protein
NCBI Official Symbol:NOP14  
NCBI Official Synonym Symbols:UTP2; NOL14; C4orf9; RES425; RES4-25  
NCBI Protein Information:nucleolar protein 14
UniProt Protein Name:Nucleolar protein 14
UniProt Synonym Protein Names:Nucleolar complex protein 14
Protein Family:Nucleolar protein
UniProt Gene Name:NOP14  
Anti-NOP14 Antibody (CAB10361)Western blot analysis of extracts of various cell lines, using NOP14 antibody (CAB10361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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