Anti-Ninein Antibody (CAB8215)
- SKU:
- CAB8215
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | NIN Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8215 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDEV EQDQ HEAR LKEL FDSF DTTG TGSL GQEE LTDL CHML SLEE VAPV LQQT LLQD NLLG RVHF DQFK EALI LILS RTLS NEEH FQEP DCSL EAQP KYVR GGKR YGRR SLPE FQES VEEF PEVT VIEP LDEE ARPS HIPA GDCS EHWK TQRS EEYE AEGQ LRFW NPDD LNAS QSGS SPPQ DWIE EKLQ EVCE DLGI TRDG HLNR KKLV SICE QYGL QNVD GEML EEVF HNLD PDGT MSVE DFFY GLFK NGKS LTPS ASTP YRQL KRHL SMQS FDES GRRT TTSS AMT |
| 遺伝子ID: | 51199 |
| Uniprot: | Q8N4C6 |
| セルラーロケーション: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 147kDa/160kDa/236-248kDa |
| 観察された分子量: | 190kDa |
| 同義語: | NIN, SCKL7, ninein |
| バックグラウンド: | This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. |
| UniProt Protein Function: | NIN: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. 9 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Microtubule-binding Chromosomal Location of Human Ortholog: 14q22.1 Cellular Component: centriole; centrosome; nucleolus; nucleus; pericentriolar material; spindle pole Molecular Function:protein binding Biological Process: centrosome localization Disease: Seckel Syndrome 7 |
| NCBI Summary: | This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8N4C6 |
| NCBI GenInfo Identifier: | 311033487 |
| NCBI Gene ID: | 51199 |
| NCBI Accession: | Q8N4C6.4 |
| UniProt Secondary Accession: | Q8N4C6,Q5BKU3, Q6P0P6, Q9BWU6, Q9C012, Q9C013, Q9C014 A6NDB8, B7WPA3, C9JSB6, C9JSG2, C9JXL2, |
| UniProt Related Accession: | Q8N4C6 |
| Molecular Weight: | 243kDa |
| NCBI Full Name: | Ninein |
| NCBI Synonym Full Names: | ninein |
| NCBI Official Symbol: | NIN |
| NCBI Official Synonym Symbols: | SCKL7 |
| NCBI Protein Information: | ninein |
| UniProt Protein Name: | Ninein |
| UniProt Synonym Protein Names: | Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein |
| Protein Family: | Ninein |
| UniProt Gene Name: | NIN |
 | Western blot analysis of extracts of Jurkat cells, using NIN antibody (CAB8215) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using NIN antibody (CAB8215) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody (CAB8215) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody (CAB8215) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using NIN antibody (CAB8215) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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