Anti-NIN Antibody (CAB12734)
- SKU:
- CAB12734
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NIN Antibody |
| 抗体コード: | CAB12734 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 380-665 of human NIN (NP_891991.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse skin, Rat skin |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 380-665 of human NIN (NP_891991.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VREK EKLR SDLD KAEK LKSL MASE VDDH HAAI ERRN EYNL RKLD EEYK ERIA ALKN ELRK EREQ ILQQ AGKQ RLEL EQEI EKAK TEEN YIRD RLAL SLKE NSRL ENEL LENA EKLA EYEN LTNK LQRN LENV LAEK FGDL DPSS AEFF LQEE RLTQ MRNE YERQ CRVL QDQV DELQ SELE EYRA QGRV LRLP LKNS PSEE VEAN SGGI EPEH GLGS EECN PLNM SIEA ELVI EQMK EQHH RDIC CLRL ELED KVRH YEKQ LDET VVSC KKAQ ENMK QRHE NETH TL |
| 遺伝子ID: | 51199 |
| Uniprot: | Q8N4C6 |
| セルラーロケーション: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 147kDa/160kDa/236-248kDa |
| 観察された分子量: | 243kDa |
| 同義語: | NIN, SCKL7, ninein |
| バックグラウンド: | This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. |
| UniProt Protein Function: | NIN: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. 9 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Microtubule-binding Chromosomal Location of Human Ortholog: 14q22.1 Cellular Component: centriole; centrosome; nucleolus; nucleus; pericentriolar material; spindle pole Molecular Function:protein binding Biological Process: centrosome localization Disease: Seckel Syndrome 7 |
| NCBI Summary: | This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8N4C6 |
| NCBI GenInfo Identifier: | 311033487 |
| NCBI Gene ID: | 51199 |
| NCBI Accession: | Q8N4C6.4 |
| UniProt Secondary Accession: | Q8N4C6,Q5BKU3, Q6P0P6, Q9BWU6, Q9C012, Q9C013, Q9C014 A6NDB8, B7WPA3, C9JSB6, C9JSG2, C9JXL2, |
| UniProt Related Accession: | Q8N4C6 |
| Molecular Weight: | 243kDa |
| NCBI Full Name: | Ninein |
| NCBI Synonym Full Names: | ninein |
| NCBI Official Symbol: | NIN |
| NCBI Official Synonym Symbols: | SCKL7 |
| NCBI Protein Information: | ninein |
| UniProt Protein Name: | Ninein |
| UniProt Synonym Protein Names: | Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein |
| Protein Family: | Ninein |
| UniProt Gene Name: | NIN |
 | Western blot analysis of extracts of various cell lines, using NIN antibody (CAB12734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using NIN antibody (CAB12734) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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