Anti-NHEJ1 Antibody (CAB19957)[KO Validated]
![Anti-NHEJ1 Antibody (CAB19957)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/14423/14310/anti-nhej1-antibody-cab19957ko-validated__38486__58051.1645802659.jpg?c=1 "Anti-NHEJ1 Antibody (CAB19957)[KO Validated]")
- SKU:
- CAB19957
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
![Anti-NHEJ1 Antibody (CAB19957)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/14423/14310/anti-nhej1-antibody-cab19957ko-validated__38486__58051.1645802659.jpg?c=1&imbypass=on "Anti-NHEJ1 Antibody (CAB19957)[KO Validated]")
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Description
| 抗体名: | Anti-NHEJ1 Antibody [KO Validated] |
| 抗体コード: | CAB19957 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human NHEJ1. |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa |
| 免疫原: | Recombinant protein of human NHEJ1. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 79840 |
| Uniprot: | Q9H9Q4 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 33kDa/35kDa |
| 観察された分子量: | 39KDa |
![Anti-NHEJ1 Antibody [KO Validated] (CAB19957)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/n/241/A19957_1__24551.jpg) | Western blot analysis of extracts from normal (control) and NHEJ1 knockout (KO) HeLa cells, using NHEJ1 antibody (CAB19957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
| 同義語: | NHEJ1, XLF |
| バックグラウンド: | Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. |
| UniProt Protein Function: | NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 2q35 Cellular Component: nucleus Molecular Function:protein binding; DNA binding Biological Process: central nervous system development; B cell differentiation; response to ionizing radiation; positive regulation of ligase activity; double-strand break repair via nonhomologous end joining; DNA recombination; T cell differentiation Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation |
| NCBI Summary: | Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H9Q4 |
| NCBI GenInfo Identifier: | 74734059 |
| NCBI Gene ID: | 79840 |
| NCBI Accession: | Q9H9Q4.1 |
| UniProt Secondary Accession: | Q9H9Q4,Q4ZFW7, Q6IA64, Q96JS9, B8ZZA4, |
| UniProt Related Accession: | Q9H9Q4 |
| Molecular Weight: | 299 |
| NCBI Full Name: | Non-homologous end-joining factor 1 |
| NCBI Synonym Full Names: | nonhomologous end-joining factor 1 |
| NCBI Official Symbol: | NHEJ1 |
| NCBI Official Synonym Symbols: | XLF |
| NCBI Protein Information: | non-homologous end-joining factor 1; XRCC4-like factor; protein cernunnos |
| UniProt Protein Name: | Non-homologous end-joining factor 1 |
| UniProt Synonym Protein Names: | Protein cernunnos; XRCC4-like factor |
| Protein Family: | Non-homologous end-joining factor |
| UniProt Gene Name: | NHEJ1 |
| UniProt Entry Name: | NHEJ1_HUMAN |