Anti-NGLY1 Antibody (CAB15864)

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SKU:
CAB15864
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-NGLY1 Antibody
抗体コード:CAB15864
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2).
申し込み:WB
推奨希釈:WB 1:500 - 1:2000
反応性:Human, Mouse, Rat
ポジティブサンプル:HeLa, Raji, Mouse brain, Mouse liver, Rat testis
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MAAA ALGS SSGS ASPA VAEL CQNT PETF LEAS KLLL TYAD NILR NPND EKYR SIRI GNTA FSTR LLPV RGAV ECLF EMGF EEGE THLI FPKK ASVE QLQK IRDL IAIE RSSR LDGS NKSH KVKS SQQP AAST QLPT TPSS NPSG LNQH TRNR QGQS SDPP SAST VAAD SAIL EVLQ SNIQ HVLV YENP ALQE KALA CIPV QELK RKSQ EKLS RARK LDKG INIS DEDF LLLE LLHW FKEE FFHW VNNV LCSK CGGQ TRSR DRSL LPSD DELK WGAK EVED HYCD ACQF SNRF PRYN NPEK
遺伝子ID:55768
Uniprot:Q96IV0
セルラーロケーション:Cytoplasm
計算された分子量:14kDa/63kDa/70kDa/72kDa/74kDa
観察された分子量:74kDa
同義語:NGLY1, CDDG, CDG1V, PNG1, PNGase
バックグラウンド:This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
NCBI Summary:This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]
UniProt Code:Q96IV0
NCBI GenInfo Identifier:13938210
NCBI Gene ID:55768
NCBI Accession:BC007226
UniProt Secondary Accession:Q96IV0,Q59FB1, Q6PJD8, Q9BVR8, Q9NR70, B4DJE9,
UniProt Related Accession:Q96IV0
Molecular Weight:70,211 Da
NCBI Full Name:Homo sapiens N-glycanase 1, mRNA
NCBI Synonym Full Names:N-glycanase 1
NCBI Official Symbol:NGLY1  
NCBI Official Synonym Symbols:CDDG; PNG1; CDG1V; PNGase  
NCBI Protein Information:peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
UniProt Protein Name:Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
UniProt Synonym Protein Names:N-glycanase 1; Peptide:N-glycanase
UniProt Gene Name:NGLY1  
Anti-NGLY1 Antibody (CAB15864)Western blot analysis of extracts of various cell lines, using NGLY1 antibody (CAB15864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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