Anti-NGF Antibody (CAB14216)
- SKU:
- CAB14216
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-NGF Antibody |
| 抗体コード: | CAB14216 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-241 of human NGF (NP_002497.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse heart, Rat brain, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-241 of human NGF (NP_002497.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EPHS ESNV PAGH TIPQ AHWT KLQH SLDT ALRR ARSA PAAA IAAR VAGQ TRNI TVDP RLFK KRRL RSPR VLFS TQPP REAA DTQD LDFE VGGA APFN RTHR SKRS SSHP IFHR GEFS VCDS VSVW VGDK TTAT DIKG KEVM VLGE VNIN NSVF KQYF FETK CRDP NPVD SGCR GIDS KHWN SYCT TTHT FVKA LTMD GKQA AWRF IRID TACV CVLS RKAV RRA |
| 遺伝子ID: | 4803 |
| Uniprot: | P01138 |
| セルラーロケーション: | Secreted |
| 計算された分子量: | 26kDa |
| 観察された分子量: | 30kDa |
| 同義語: | NGF, Beta-NGF, HSAN5, NGFB |
| バックグラウンド: | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. |
| UniProt Protein Function: | NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: cytoplasmic membrane-bound vesicle; endosome; extracellular region; Golgi lumen Molecular Function:growth factor activity; metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding; receptor signaling protein activity Biological Process: activation of MAPKK activity; cell-cell signaling; induction of apoptosis via death domain receptors; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of neuron apoptosis; nerve growth factor processing; nerve growth factor receptor signaling pathway; neurite morphogenesis; neuron apoptosis; peripheral nervous system development; phosphoinositide-mediated signaling; phospholipase C activation; positive regulation of apoptosis; positive regulation of axon extension; positive regulation of axonogenesis; positive regulation of collateral sprouting; positive regulation of neuron maturation; positive regulation of protein ubiquitination; Ras protein signal transduction; regulation of axonogenesis; regulation of caspase activity; regulation of neuron differentiation; regulation of neurotransmitter secretion; regulation of release of sequestered calcium ion into cytosol; sensory perception of pain; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V |
| NCBI Summary: | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P01138 |
| NCBI GenInfo Identifier: | 90110037 |
| NCBI Gene ID: | 4803 |
| NCBI Accession: | P01138.3 |
| UniProt Secondary Accession: | P01138,Q6FHA0, Q96P60, Q9P2Q8, Q9UKL8, A1A4E5, |
| UniProt Related Accession: | P01138 |
| Molecular Weight: | 26,959 Da |
| NCBI Full Name: | Beta-nerve growth factor |
| NCBI Synonym Full Names: | nerve growth factor |
| NCBI Official Symbol: | NGF |
| NCBI Official Synonym Symbols: | NGFB; HSAN5; Beta-NGF |
| NCBI Protein Information: | beta-nerve growth factor |
| UniProt Protein Name: | Beta-nerve growth factor |
| Protein Family: | Nerve growth factor |
| UniProt Gene Name: | NGF |
| UniProt Entry Name: | NGF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NGF antibody (CAB14216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat breast using NGF Rabbit pAb (CAB14216) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-251MG cells using NGF antibody (CAB14216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |