Anti-NFKBIE Antibody (CAB8443)
- SKU:
- CAB8443
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NFKBIE Antibody |
| 抗体コード: | CAB8443 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human NFKBIE (NP_004547.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse |
| ポジティブサンプル: | Mouse liver, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human NFKBIE (NP_004547.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSEA RKGP DEAE ESQY DSGI ESLR SLRS LPES TSAP ASGP SDGS PQPC THPP GPVK EPQE KEDA DGER ADST YGSS SLTY TLSL LGGP EAED PAPR LPLP HVGA LSPQ QLEA LTYI SEDG DTLV HLAV IHEA PAVL LCCL A |
| 遺伝子ID: | 4794 |
| Uniprot: | O00221 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 52kDa |
| 観察された分子量: | 60kDa |
| 同義語: | NFKBIE, IKBE |
| バックグラウンド: | The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. |
| UniProt Protein Function: | IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family. |
| UniProt Protein Details: | Protein type:DNA-binding; Inhibitor Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding Biological Process: cytoplasmic sequestering of transcription factor |
| NCBI Summary: | The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011] |
| UniProt Code: | O00221 |
| NCBI GenInfo Identifier: | 206729919 |
| NCBI Gene ID: | 4794 |
| NCBI Accession: | O00221.3 |
| UniProt Secondary Accession: | O00221,Q5T9V9, |
| UniProt Related Accession: | O00221 |
| Molecular Weight: | 52,864 Da |
| NCBI Full Name: | NF-kappa-B inhibitor epsilon |
| NCBI Synonym Full Names: | NFKB inhibitor epsilon |
| NCBI Official Symbol: | NFKBIE |
| NCBI Official Synonym Symbols: | IKBE |
| NCBI Protein Information: | NF-kappa-B inhibitor epsilon |
| UniProt Protein Name: | NF-kappa-B inhibitor epsilon |
| UniProt Synonym Protein Names: | I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIE |
| UniProt Entry Name: | IKBE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NFKBIE antibody (CAB8443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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