Anti-NF2 Antibody (CAB13626)[KO Validated]
![Anti-NF2 Antibody (CAB13626)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/19997/19857/anti-nf2-antibody-cab13626ko-validated__74924__50200.1645804447.jpg?c=1 "Anti-NF2 Antibody (CAB13626)[KO Validated]")
- SKU:
- CAB13626
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
![Anti-NF2 Antibody (CAB13626)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/19997/19857/anti-nf2-antibody-cab13626ko-validated__74924__50200.1645804447.jpg?c=1&imbypass=on "Anti-NF2 Antibody (CAB13626)[KO Validated]")
Description
| 抗体名: | Anti-NF2 Antibody [KO Validated] |
| 抗体コード: | CAB13626 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1). |
| 申し込み: | WB IP |
| 推奨希釈: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TKPT YPPM NPIP APLP PDIP SFNL IGDS LSFD FKDT DMKR LSME IEKE KVEY MEKS KHLQ EQLN ELKT EIEA LKLK ERET ALDI LHNE NSDR GGSS KHNT |
| 遺伝子ID: | 4771 |
| Uniprot: | P35240 |
| セルラーロケーション: | Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane |
| 計算された分子量: | 19-30kDa/59-72kDa |
| 観察された分子量: | 70KDa |
| 同義語: | NF2, ACN, BANF, SCH, merlin |
| バックグラウンド: | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. |
| UniProt Protein Function: | Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts. |
| UniProt Protein Details: | Protein type:Tumor suppressor; Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: adherens junction; apical part of cell; cleavage furrow; cortical actin cytoskeleton; cytoplasm; cytoskeleton; early endosome; extrinsic to membrane; filopodium membrane; lamellipodium; lipid raft; membrane; neuron projection; nucleolus; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; synapse Molecular Function:actin binding; beta-catenin binding; integrin binding; protein binding; protein domain specific binding Biological Process: actin cytoskeleton organization and biogenesis; ectoderm development; hippocampus development; intercellular junction assembly and maintenance; mesoderm formation; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of cell-matrix adhesion; negative regulation of DNA replication; negative regulation of JAK-STAT cascade; negative regulation of MAPKKK cascade; negative regulation of protein kinase activity; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of tyrosine phosphorylation of Stat5 protein; odontogenesis of dentine-containing teeth; positive regulation of cell differentiation; positive regulation of stress fiber formation; regulation of gliogenesis; regulation of protein stability; Schwann cell proliferation; small GTPase mediated signal transduction Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1 |
| NCBI Summary: | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35240 |
| NCBI GenInfo Identifier: | 462594 |
| NCBI Gene ID: | 4771 |
| NCBI Accession: | P35240.1 |
| UniProt Secondary Accession: | P35240,O95683, Q8WUJ2, Q969N0, Q969Q3, Q96T30, Q96T31 Q96T32, Q96T33, Q9BTW3, Q9UNG9, Q9UNH3, |
| UniProt Related Accession: | P35240 |
| Molecular Weight: | 24,515 Da |
| NCBI Full Name: | Merlin |
| NCBI Synonym Full Names: | neurofibromin 2 (merlin) |
| NCBI Official Symbol: | NF2 |
| NCBI Official Synonym Symbols: | ACN; SCH; BANF |
| NCBI Protein Information: | merlin |
| UniProt Protein Name: | Merlin |
| UniProt Synonym Protein Names: | Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin |
| Protein Family: | Merlin |
| UniProt Gene Name: | NF2 |
| UniProt Entry Name: | MERL_HUMAN |
![Anti-NF2 Antibody [KO Validated] (CAB13626)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/i/512/A13626_1__61589.jpg) | Western blot analysis of extracts of HepG2 cells, using NF2 Rabbit pAb (CAB13626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
![Anti-NF2 Antibody [KO Validated] (CAB13626)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/p/202/A13626_2__54485.jpg) | Western blot analysis of extracts from normal (control) and NF2 Rabbit pAb knockout (KO) HeLa cells, using NF2 Rabbit pAb antibody (CAB13626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
![Anti-NF2 Antibody [KO Validated] (CAB13626)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/h/108/A13626_3__97719.jpg) | Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug NF2 antibody (CAB13626). Western blot was performed from the immunoprecipitate using NF2 antibody (CAB13626) at a dilition of 1:1000. |