Anti-Neurocalcin-delta Antibody (CAB8000)
- SKU:
- CAB8000
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | NCALD Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8000 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human NCALD (NP_114430.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-549, THP-1, BT-474, Mouse brain, Mouse liver, Mouse lung, Mouse spinal cord, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human NCALD (NP_114430.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGKQ NSKL RPEV MQDL LEST DFTE HEIQ EWYK GFLR DCPS GHLS MEEF KKIY GNFF PYGD ASKF AEHV FRTF DANG DGTI DFRE FIIA LSVT SRGK LEQK LKWA FSMY DLDG NGYI SKAE MLEI VQAI YKMV SSVM KMPE DEST PEKR TEKI FRQM DTNR DGKL SLEE FIRG AKSD PSIV RLLQ CDPS SAGQ F |
| 遺伝子ID: | 83988 |
| Uniprot: | P61601 |
| セルラーロケーション: | |
| 計算された分子量: | 22kDa |
| 観察された分子量: | 22kDa |
| 同義語: | NCALD |
| バックグラウンド: | This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. |
| UniProt Protein Function: | NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol Molecular Function:tubulin binding; protein binding; clathrin binding; calcium ion binding; actin binding; alpha-tubulin binding Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling |
| NCBI Summary: | This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61601 |
| NCBI GenInfo Identifier: | 47606440 |
| NCBI Gene ID: | 83988 |
| NCBI Accession: | P61601.2 |
| UniProt Related Accession: | P61601 |
| Molecular Weight: | |
| NCBI Full Name: | Neurocalcin-delta |
| NCBI Synonym Full Names: | neurocalcin delta |
| NCBI Official Symbol: | NCALD |
| NCBI Protein Information: | neurocalcin-delta |
| UniProt Protein Name: | Neurocalcin-delta |
| Protein Family: | Neurocalcin |
| UniProt Gene Name: | NCALD |
| UniProt Entry Name: | NCALD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCALD antibody (CAB8000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 cells using NCALD antibody (CAB8000). Blue: DAPI for nuclear staining. |
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