Anti-NEK8 Antibody (CAB0984)
- SKU:
- CAB0984
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NEK8 Antibody |
| 抗体コード: | CAB0984 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, Jurkat, K-562, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEKY ERIR VVGR GAFG IVHL CLRK ADQK LVII KQIP VEQM TKEE RQAA QNEC QVLK LLNH PNVI EYYE NFLE DKAL MIAM EYAP GGTL AEFI QKRC NSLL EEET ILHF FVQI LLAL HHVH THLI LHRD LKTQ NILL DKHR MVVK IGDF GISK ILSS KSKA YTVV GTPC YISP ELCE GKPY KNVS SATH SACV LYEL ASLK RAFE AANL PALV LKIM SGTF APIS DRYS PELR QLVL SLLS LEPA QRPP LSHI MAQP LCIR ALLN LHTD VGSV RMRR AEKS VAPS NTGS RTTS VRCR GIPR |
| 遺伝子ID: | 284086 |
| Uniprot: | Q86SG6 |
| セルラーロケーション: | Cell projection, Cytoplasm, cilium, cytoskeleton |
| 計算された分子量: | 74kDa |
| 観察された分子量: | 80kDa |
| 同義語: | NEK8, JCK, NEK12A, NPHP9, RHPD2 |
| バックグラウンド: | This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. |
| UniProt Protein Function: | NEK8: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. May play a role in mitotic regulation.Required for renal tubular integrity. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family Chromosomal Location of Human Ortholog: 17q11.1 Cellular Component: cytoskeleton; cytoplasm Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding Biological Process: organ morphogenesis; mitotic cell cycle; protein amino acid phosphorylation; microtubule-based process Disease: Renal-hepatic-pancreatic Dysplasia 2; Nephronophthisis 9 |
| NCBI Summary: | This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q86SG6 |
| NCBI GenInfo Identifier: | 34098463 |
| NCBI Gene ID: | 284086 |
| NCBI Accession: | Q86SG6.1 |
| UniProt Secondary Accession: | Q86SG6,Q14CL7, Q2M1S6, Q8NDH1, A6NIC5, |
| UniProt Related Accession: | Q86SG6 |
| Molecular Weight: | Calculated MW: 74kDaObserved MW: 80kDa |
| NCBI Full Name: | Serine/threonine-protein kinase Nek8 |
| NCBI Synonym Full Names: | NIMA-related kinase 8 |
| NCBI Official Symbol: | NEK8 |
| NCBI Official Synonym Symbols: | JCK; NPHP9; RHPD2; NEK12A |
| NCBI Protein Information: | serine/threonine-protein kinase Nek8; nephrocystin 9; NIMA-family kinase NEK8; NIMA-related kinase 12a; nimA-related protein kinase 8; nima-related protein kinase 12a; never in mitosis A-related kinase 8; serine/thrionine-protein kinase NEK8; NIMA (never in mitosis gene a)- related kinase 8 |
| UniProt Protein Name: | Serine/threonine-protein kinase Nek8 |
| UniProt Synonym Protein Names: | Never in mitosis A-related kinase 8; NimA-related protein kinase 8; Nima-related protein kinase 12a |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | NEK8 |
| UniProt Entry Name: | NEK8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEK8 antibody (CAB0984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using NEK8 antibody (CAB0984). Blue: DAPI for nuclear staining. |
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